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| The relationship between the expression of miR-155 in peripheral blood and the differentiation of CD8+T cells in patients with pulmonary tuberculosis |
| WEI Liancun |
| Department of Laboratory, the Fourth People′s Hospital of Qinghai Province, Qinghai Province, Xi′ning 810000, China |
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Abstract Objective To investigate the relationship between the expression of microRNA-155 (miR-155) in peripheral blood mononuclear cells (PBMC) and the differentiation of CD8+T cells in patients with pulmonary tuberculosis. Methods A total of 88 patients with pulmonary tuberculosis infection who admitted to the Fourth People′s Hospital of Qinghai Province from January 2017 to June 2019. Among them, 42 cases were active pulmonary tuberculosis (active group), 46 cases were latent tuberculosis infection (latent group), and 50 cases were healthy during the same period as control group. The expressions of miR-155, T lymphocyte subsets in PBMC and interferon-γ (IFN-γ), interleukin-4 (IL-4) and IFN-γ/IL-4 in three groups were observed, the levels of IFN-γ, IL-4 and IFN-γ/IL-4 in CD8+T cells of patients with different miR-155 expression were analyzed. Results The expressions of miR-155, CD3+CD8+, IFN-γ and IL-4 in PBMC of the active group and latent group were higher than those of the control group, and the activity group was higher than the latent group (P < 0.05), the IFN-γ/IL-4 ratio in the active group was higher than that in the control group (P < 0.05). The percentage of PBMC CD3+CD4+ cells and CD4+/CD8+ ratio in active group and latent group were lower than those in control group, and the active group were lower than those in latent group (P < 0.05). The ratios of IFN-γ, IL-4 and IFN-γ/IL-4 in the high expression group of miR-155 was higher than that of the low expression group of miR-155 (P < 0.05). The expression of PBMC miR-155 was positively correlated with IFN-γ, IL-4 and IFN-γ/IL-4 ratio (r = 0.695, P = 0.000; r = 0.477, P = 0.000; r = 0.446, P = 0.000). Conclusion PBMC miR-155 is highly expressed in patients with pulmonary tuberculosis, which induces activation of CD8+T cells and differentiation into cytotoxic T ceu 1 phenotype.
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