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| Expression of serum nerve growth factor and cellular immunity in breast cancer and its correlation with clinicopathological feature |
| WANG Wenhui1 WEN Yuanyuan2 CHEN Hao1 |
1.Department of Pathology, Hangzhou Maternity Hospital, Zhejiang Province, Hangzhou 310008, China;
2.Pathological Diagnosis Center, Zhejiang Zhoushan Hospital, Zhejiang Province, Zhoushan 316021, China |
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Abstract Objective To investigate the expression of serum nerve growth factor (NGF) and cellular immunity in breast cancer patients and its correlation with clinicopathological feature. Methods A total of 49 breast cancer patients diagnosed in Hangzhou Maternity Hospital and Zhejiang Zhoushan Hospital from March 2015 to February 2018 were selected as the observation group. A total of 45 patients with benign breast disease who were hospitalized at the same time were selected as the control group. A total of 47 female patients with physical examination at the same time were selected as the normal group. The expression levels of NGF in each group were determined by enzyme-linked immunosorbent assay. The levels of CD3+, CD4+, CD8+ and CD4+/CD8+ in each group were determined by flow cytometry. The clinicopathological data of the patients were recorded and counted. The levels of NGF and cellular immunity in each group and the levels of NGF and cellular immunity in different clinicopathological data of breast cancer in the observation group were analyzed. The correlation between NGF, cellular immunity level and clinicopathological data of breast cancer patients was analyzed by Pearson analysis. Results The levels of NGF and cellular immunity in the three groups were statistically significant (P ＜ 0.05). The level of NGF in the observation group was higher than that in the control group and the normal group, and the level of NGF in the control group was higher than that in the normal group, with statistically significant differences (P ＜ 0.05). The levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were lower than those in the control group and the normal group, and the level of CD8+ was higher than that in the control group and the normal group, with statistically significant differences (P ＜ 0.05). There was no significant difference in the levels of CD3+, CD4+, CD8+ and CD4+/CD8+ between the control group and the normal group (P ＞ 0.05). The levels of NGF, CD3+, CD4+, CD8+ and CD4+/CD8+ in breast cancer tissues were not statistically significant differences in terms of age and histopathological type (P ＞ 0.05). The levels of NGF, CD3+, CD4+, CD8+ and CD4+/CD8+ in breast cancer tissues were compared in terms of tumor maximum diameter, pathological grading, differentiation degree and lymph node metastasis, with statistically significant differences (P ＜ 0.05). The Pearson correlation analysis results showed that the NGF and CD8+ levels were positively correlated with the maximum tumor diameter, pathological grading, differentiation degree and lymph node metastasis (P ＜ 0.05). The levels of CD3+, CD4+ and CD4+/CD8+ were negatively correlated with the maximum tumor diameter, pathological grading, differentiation degree and lymph node metastasis (P ＜ 0.05). Conclusion The high expression of NGF in breast cancer patients leads to the decline of cellular immunity level in the body, and the expression level of both is correlated with the pathology feature. Strengthening the determination of NGF and cellular immunity level can assess the severity of the disease in patients and guide clinical treatment.
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