siRNA干扰Annexin A7对宫颈癌细胞株Ki67和P16的影响

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Abstract Objective To study the effect of Annexin A7 expression inhibition on Ki67 and P16 of Hela cells. Methods After cell culture, the cells were divided into the siRNA interference group, the negative control group and the blank control group for small interfering RNA (siRNA) interference to inhibit the expression of Annexin A7 in Hela cells of cervical cancer cell line. After the interference efficiency was identified by Western blot, the effect of the inhibition of Annexin A7 on the expression of Ki67 and P16 in Hela cells was detected by laser confocal immunofluorescence assay. Results Annexin A7 expression in the siRNA intervention group was significantly lower than that in the negative control group and the blank control group, with statistically significant difference (P ＜ 0.05). Compared with the negative control group and the blank control group, Ki67 positive products were significantly reduced in the siRNA interference group, and the difference was statistically significant (P ＜ 0.05). There was no statistically significant difference in P16 positive products among the siRNA interference group, the negative control group and the blank control group (P ＞ 0.05). Conclusion Annexin A7 may get involved in upregulated cell proliferation of squamous cell carcinoma of the cervix.

Key words： Annexin A7 Ki67 P16 Cervical carcinoma

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	ZHOU Xing1 CHEN Xue1 CUI Lei2 SUN Jirui1 ZHANG Bingxin1 LI Yixuan1 ZHANG Jinku1▲

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ZHOU Xing1 CHEN Xue1 CUI Lei2 SUN Jirui1 ZHANG Bingxin1 LI Yixuan1 ZHANG Jinku1▲. Effect of siRNA interference Annexin A7 on cervical cancer cell lines Ki67 and P16[J]. 中国医药导报, 2020, 17(11): 12-14.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I11/12

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