艾菲提蒙汤对动脉粥样硬化家兔血管平滑肌迁移和增殖相关蛋白的影响

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Abstract Objective To investigate the effect of Aifeitimeng Tang on vascular smooth cell migration and proliferation. Methods Forty-eight rabbits were randomly divided into normal group, model group, positive control group and Aifeitimeng Tang high, medium and low dose groups, with 8 rabbits in each group. Normal group was given normal feed. Other 5 groups were fed high-fat diet, once a day for 60 consecutive days. Model group was gavaged with normal saline, Simvastatin Tablets in positive control group were gavaged with 0.002 g/kg and Aifeitimeng Tang was gavaged with low, medium and high dose groups with 3.3, 6.5, 13.0 g/kg, once a day, for 60 consecutive days. Immunohistochemistry was used to determine the effect of Aifeitimeng Tang on proliferating cell nuclear antigen (PCNA) and platelet derived growth factor (PDGF) in rabbits with AS, PCNA protein expression was detected by Western blot. Results In normal group, a small number of PCNA positive cells were observed, and a small amount of uneven brown-yellow granules were observed in the nucleus. The percentage of PCNA positive cells in model group was higher than that in normal group (P < 0.01). The percentage of PCNA positive cells in Aifeitimeng Tang high, medium and low dose groups were lower than those in model group (P < 0.05 or P < 0.01). A small number of PDGF positive cells were observed in normal group, and a small amount of uneven brown-yellow granules were observed in the nucleus. The percentage of PDGF positive cells in model group was higher than that in normal group (P < 0.05), and the percentage of PDGF positive cells in Aifeitimeng Tang low and high dose groups were lower than those in model group (P < 0.05). Conclusion Aifeitimeng Tang has a significant inhibitory effect on the formation of experimental rabbits model of AS, and its mechanism is related to the decrease of PCNA and PDGF content in the vascular wall and the decrease of PCNA protein expression in the vascular wall of rabbits.

Key words： Aifeitimeng Tang Rabbits Athemsclerosis Proliferating cell nuclear antigen Platelet derived growth factor

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	Articles by authors
	Haiernishaguli·Yiming1
	2 Mireguli·Kmili3 DOU Qin2 LI Zhijian2 Silafu·Aibai1
	2

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Haiernishaguli·Yiming1,2 Mireguli·Kmili3 DOU Qin2 LI Zhijian2 Silafu·Aibai1,2. Effect of Aifeitimeng Tang on vascular smooth cell migration and proliferation-related proteins in rabbits with atherosclerosis[J]. 中国医药导报, 2020, 17(11): 4-7.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I11/4

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