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| Repair effect of placental mesenchymal stem cells on endometrial injury in rats |
| WANG Xiangxiang1,2 WANG Yan1,2 YANG Shaoshao1,2 ZHANG Junjun2 |
1.College of Clinical Medical, He′nan University of Science and Technology, He′nan Province, Luoyang 471003, China;
2.Department of Obstetrics and Gynecology, the First Affiliated Hospital of He′nan University of Science and Technology, He′nan Province, Luoyang 471003, China |
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Abstract Objective To study the repair effect of placental mesenchymal stem cells on endometrial injury in rats. Methods From May to November 2019, a total of 60 female SD rats (Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing) were constructed endometrial injury models by chemical damage method, and they were divided into control group, gel group and model group by simple random sampling, with 20 rats in each group. After three estrous cycles, 10 rats from each group were randomly sampled for HE staining, and the number of endometrial glands and endometrial thickness were measured, while the percentage of endometrial fibrosis were measured by Masson staining. The other 30 rats were caged with male rats. On the 10th day of conception, the number of uterine embryos in the rats was observed by laparotomy. Results Compared with the control group, the number of endometrial glands decreased, the thickness of the endometrium became thinner, the percentage of endometrial fibrosis increased, while the number of uterine embryos in pregnant rats decreased in the model group, and the differences were statistically significant (P ＜ 0.05). Compared with the model group, the number of endometrial glands increased, the thickness of the endometrium increased, the percentage of endometrial fibrosis area decreased, while the number of uterine embryos in pregnant rats increased in the gel group, and the differences were statistically significant (P ＜ 0.05). Compared with the control group, the number of endometrial glands and endometrial thickness had no significant changes in the gel group, and the differences were not statistically significant (P ＞ 0.05); while the percentage of endometrial fibrosis increased, the number of uterine embryos in pregnant rats decreased, and the differences were statistically significant (P ＜ 0.05). Conclusion Placental mesenchymal stem cells can repair damaged endometrium, increase the number of endometrial glands and endometrial thickness, reduce the percentage of endometrial fibrosis area, and improve embryo implantation rate.
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