|
|
|
| Experimental study on the effect of acupoint application of Jingyi Anshen Powder on neurotransmitters in rats with insomnia |
| HU Jinxia1 LU Jun2 WANG Hongxia1 JIANG Yu1 LIU Tao1 HU Xiaoling1 |
| 1.Department of Geriatrics, Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region, Urumqi 830000, China; 2.Department of Pharmacy, Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region, Urumqi 830000, China |
|
|
|
Abstract Objective To explore the effect of acupoint application of Jingyi Anshen Powder on neurotransmitters in rats with insomnia. Methods According to the random number table method, 64 male rats were divided into four groups: control group (n = 16), model group (n = 16), blank group (n = 16) and experimental group (n = 16). Control group was intraperitoneally injected with 0.9% Sodium Chloride Solution (1 mL/100 g) once a day for 2 days, and fed continuously for 7 days without any intervention. Model group, blank group and experimental group were all injected intraperitoneally with P-phenylalanine (PCPA) once a day for two consecutive days to create a sleep deprivation model. After molding, model group was fed for 7 days without any intervention. After molding, in blank group, blank film was applied to shenque and neiguan acupoints once a day, 30 minutes a time for 7 days. After molding, in experimental group, Jingyi Anshen Powder was applied to shénquē and nèigu?觀n acupoints once a day for 30 minutes for 7 days. On the 8th day of the experiment, the rats were decapitated, the brain tissues were taken out after craniotomy, the hypothalamus was separated, washed and chopped, and the supernatant was taken from the homogenate. Contents of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), dopamine (DA) and noradrenaline (NANE) were measured by enzyme-linked immunosorbent assay. Results The exhaustion time of model group, blank group and experimental group was shorter than that of control group, and the despair time of model group, blank group and experimental group was longer than that of control group, the differences were statistically significant (P < 0.01). The exhaustion time of blank group and experimental group was longer than that of model group, and the desperation time of blank group and experimental group was shorter than that of model group, the differences were highly statistically significant (P < 0.01). The contents of 5-HT in model group and blank group were lower than that in control group, the contents of 5-HT in experimental group was higher than that in model group and blank group, the differences were statistically significant (P < 0.05 or P < 0.01). The contents of 5-HIAA in model group and blank group were lower than that in control group, while the content of 5-HIAA in experimental group was higher than that in model group, the differences were highly statistically significant (P < 0.01). DA contents of hypothalamus in model group and blank group were higher than that in control group, DA content of hypothalamus in experimental group was lower than that in model group, the differences were highly statistically significant (P < 0.01). The contents of hypothalamic NANE in model group and blank group were higher than that in control group, the contents of hypothalamic NANE in experimental group were lower than that in model group and blank group, the differences were statistically significant (P < 0.05 or P < 0.01). Conclusion Jingyi Anshen Powder is applied to the acupoints of insomnia rats to improve sleep by affecting the changes of the level of neurotransmitters in the hypothalamus.
|
|
|
|
|
|
[1] 魏清，胡金霞，胡晓灵.景衣安神散穴位贴敷治疗失眠临床观察[J].吉林中医药，2012，32（33）：299-300.
[2] 胡金霞，杨帆.穴位贴敷疗法治疗更年期综合征失眠症临床观察[J].时珍国医国药，2013，24（6）：1445-1446.
[3] 乔飞，胡晓灵，胡金霞，等.景衣安神散穴位贴敷治疗失眠临床观察[J].山西中医，2010，26（4）：37-38.
[4] 胡金霞，张磊，魏清，等.应用新疆地产中药穴位贴敷治疗不寐患者的临床疗效观察[J].新疆中医药，2013，31（4）：36-39.
[5] 胡金霞，张磊，胡晓灵，等.应用中药穴位贴敷治疗不寐的临床疗效观察[J].光明中医，2013，28（10）：2103-2105.
[6] 郭鑫，岳增辉，谢菊英，等.针刺对失眠大鼠血清去甲肾上腺素、多巴胺及5-羟色胺含量的影响[J].中国中医药信息杂志，2018，25（4）：46-50.
[7] 赵仁云，郑竹宏，丁玉婷，等.栀子厚朴汤对失眠模型小鼠行为学及脑内单胺类神经递质的影响[J].国际药学研究杂志，2018，6（45）：436-442.
[8] 全世建，林杏娥，刘妮.PCPA大鼠失眠模型的证候属性研究[J].中医药学刊，2006，24（3）：450-451.
[9] 方福德，周吕，丁濂，等.现代医学实验技巧全书[M].北京：北京医科大学中国协和医科大学联合出版社，1995：12.
[10] 华兴邦，周浩良.大鼠穴位图谱的研制[J].实验动物与动物实验，1991（l）：1-5.
[11] 游秋云，王平，田代志，等.老年肝郁失眠证候大鼠模型的建立和评价[J].中国实验方剂学杂志，2013，19（2）：222-225.
[12] 路腾飞，于泽胜，姚璐，等.柴胡白芍药对对慢性温和不可预知性应激抑郁模型大鼠行为学的影响[J].中草药，2016，47（12）：2137-2141.
[13] 鲍秋荔.红景天药理作用研究进展[J].饮食保健，2019， 6（8）：298-299.
[14] 古丽妮尕尔·阿布力克木，尼格尔热依·亚迪卡尔，阿吉艾克拜尔·艾萨，等.薰衣草残渣化学成分及其生物活性[J].中成药，2019，41（4）：818-822.
[15] 王芳，于姚，毛水先，等.穴位贴敷疗法治疗失眠的关键问题探讨[J].中国中医基础医学杂志，2018，24（1）：104-107.
[16] 赵辉，白玉彦，温桂莲.氢溴酸西泰普兰片联合针刺治疗PSD的疗效及对神经免疫验症指标的影响[J].重庆医学，2018，47（14）：1952-1957.
[17] Wang X，Yang B，Sun H，et al. Pattern recognition approaches and computational systems tools for ultraperformance liquid chmmatography mass spectrometry-based comprehensive metabolomic profiling and pathways analysis of biological data sets [J]. Anal Chem，2012，84（1）：428-439．
[18] 张国炳，陈峻严.脑内多巴胺受体的分布和功能[J].中国校医，2013，27（1）：38-40.
[19] Riemann D. Non-pharmacological treatment of insomnia [J]. Ther Umsch，2014，71（11）：687-694.
[20] 石皓月，鲁艺，李钰昕，等.中药治疗对氯苯丙氨酸失眠模型大鼠影响的基础研究进展[J].中国医药导报，2018， 15（11）：33-36.
[21] Gao J，Zhang JX，Xu TL. Modulation of serotonergic projection from dorsal raphe nucleus to baso later alamygdaline sleep-waking cycle of rats [J]. Brain Res，2002，94（5）：60-70.
[22] 成光宇，丁云录，赵德喜，等.和解安神汤对肝郁脾虚证候失眠大鼠模型的影响[J].中国老年学杂志，2017，37（13）：3160-3162. |
|
|
|
