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| Changes and clinical significance of serum carbohydrate response element binding protein in patients with diabetic nephropathy |
| HAN Bing1 CHEN Ye2 QI Li2 |
| 1.Department of Nephrology, the Second People′s Hospital and Gynecology and Obstetrics Hospital of Fuxin City, Liaoning Province, Fuxin 123000, China; 2.Department of Nephrology, Fuxin Central Hospital, Liaoning Province, Fuxin 123000, China |
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Abstract Objective To study the changes of serum carbohydrate response element binding protein(ChREBP) level in patients with type 2 diabetes mellitus (T2DM) and its relationship with diabetic nephropathy (DN). Methods From February 2016 to December 2018, 193 cases with T2DM patients who admitted to the Second People′s Hospital and Gynecology and Obstetrics Hospital of Fuxin City of Liaoning Province (“our hospital” for short) were selected. According to the results of urinary albumin creatinine ratio (UACR), the patients whose UACR≤30 were in simple T2DM group (123 cases), and the patients with UACR>30 were in DN group (70 cases), and 50 healthy people receiving physical examination in our hospital during the same period were selected as control group. Clinical data in three group were compared. The serum ChREBP level was detected by enzyme-linked immunosorbent assay and logistic regression analysis was conducted DN influencing factors. The energy efficiency of serum ChREBP in the diagnosis of DN was analyzed by the receiver operating characteristic curve of subjects. Results The duration of diabetes in DN group was longer than that of simple T2DM group (P < 0.05). The levels of fasting blood-glucose (FBG), glycosylated hemoglobin (HbA1c), total cholesterol (TC)，triglyceride (TG) and low density lipoprotein cholesterin (LDL-C) in simple T2DM group and DN group were higher than those of control group, while the level of high density lipoprotein cholesterol (HDL-C) was lower than that of control group (all P < 0.05). The 24 h urinary albumin excretion rate (24 h UAER) and ChREBP levels of DN group were higher than those of control group and simple T2DM group, and the glomerular filtration rate (eGFR) was lower than that of control group and simple T2DM group (all P < 0.05). The levels of 24 h UAER and ChREBP insimple T2DM group were higher than those in control group, and the eGFR was lower than that in control group (all P < 0.05). Duration of diabetes, 24 h UAER and ChREBP levels were independent risk factors for DN. The cut-off value was 154.36 ng/mL, the sensitivity was 0.872, and the specificity was 0.834. Conclusion Serum ChREBP level is abnormally highly expressed in DN patients, and clinical detection of serum ChREBP level can be used for early diagnosis of DN.
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