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| Changes and clinical significance of serum miR-1-3p and H-FABP levels in patients with acute ST-segment elevation myocardial infarction |
| ZHANG Rongfeng LI Danna DONG Yingxue |
| Department of Cardiology, the First Affiliated Hospital of Dalian Medical University, Liaoning Province, Dalian 116011, China |
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Abstract Objective To investigate the expression of serum miR-1-3p and Heart-type fatty acid binding protein (H-FABP) in patients with acute ST-segment elevation myocardial infarction (STEMI), and to analyze the diagnostic value of both to STEMI. Methods A total of 120 STEMI patients treated in the First Affiliated Hospital of Dalian Medical University (hereinafter referred to as “our hospital”) from December 2016 to June 2019 were selected as the STEMI group, and another 120 patients with normal cardiac function in our hospital during the same period were selected as the non-STEMI group. The expression of serum miR-1-3p was detected by real-time fluorescence quantitative PCR. Serum levels of H-FABP and N-terminal pro B-type natriuretic peptide (NT-proBNP) were determined by enzyme-linked immunosorbent assay. The levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and triacylglycerol (TG) were measured by automatic biochemical analyzer. Left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD) were measured by color ultrasound diagnostic instrument. Pearson correlation was used to analyze the correlation of serum miR-1-3p and H-FABP with lipid metabolism index and cardiac function index in STEMI patients. The diagnostic value of serum miR-1-3p, H-FABP and combined detection of STEMI was analyzed by using the receiver operating characteristic curve (ROC). Results Serum miR-1-3p expression and LVEF in the STEMI group were lower than those in the non-STEMI group, and the levels of serum H-FABP, TC, LDL-C, NT-proBNP and LVEDD in STEMI group were higher than those in the non-STEMI group, with statistically significant differences (all P ＜ 0.05). There was no significant difference in serum TG between the two groups (P ＞ 0.05). Serum miR-1-3p in STEMI group was negatively correlated with TC, LDL-C, NT-proBNP, LVEDD, and H-FABP (r ＜ 0, P ＜ 0.05), and positively correlated with LVEF (r ＞ 0, P ＜ 0.05). Serum H-FABP was positively correlated with TC, LDL-C, NT-proBNP and LVEDD (r ＞ 0, P ＜ 0.05), and negatively correlated with LVEF and miR-1-3p (r ＜ 0, P ＜ 0.05). There was no significant correlation between them and TG (P > 0.05). ROC analysis showed that serum miR-1-3p and H-FABP had certain diagnostic value for STEMI, and the diagnostic value of the combined detection was higher than that of the single detection [AUC=0.815, 95%CI (0.728-0.846)]. Conclusion Serum miR-1-3p was low expressed and H-FABP was high expressed in STEMI patients, both of which were correlated with lipid metabolism index and cardiac function index of the patients to some extent. The combined detection of the two could further improve the diagnostic value of STEMI.
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