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| Effect of acupuncture needle and round sharp needle on β-endorphin and prostaglandin E2 in rats with knee osteoarthritis |
| LI Huijuan1 ZHANG Dong2 ZHANG Xiaozhe2 RUAN Anmin2 CHEN Pu2 ZHOU Jun2 FAN Xiaoyan2 WANG Qingfu1 |
| 1.Department of Orthopedics of Traditional Chinese Medicine, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China; 2.Department of Orthopedics of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China |
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Abstract Objective To explore effect of acupuncture needle and round sharp needle on β-endorphin and prostaglandin E2 (PGE2) in rats with knee osteoarthritis (KOA). Methods KOA model of rats was established by injecting papain into the joint cavity. 40 male Wistar rats were randomly divided into normal group, model group, acupuncture needle group and round sharp needle group, with 10 rats in each group. Needle knife intervention was used in needle knife group, and needle knife intervention with the same diameter was used in the round needle knife group. Both interventions were treated with needle knife technique once a week for 4 weeks. Behavior of rat in each group before and after intervention was evaluated. Serum levels of β-endorphin and PGE2 were measured after sampling. Results Before intervention, behavioral scores of Lequesne MG in model group, acupuncture needle group and round sharp needle group were higher than those in normal group (P < 0.01). After intervention, behavioral scores of Lequesne MG in model group, acupuncture needle group and round sharp needle group were higher than those in normal group (P < 0.01). The Lequesne MG behavioral scores after intervention in model group, acupuncture needle group and round sharp needle group were lower than those before intervention, there were highly statistically significant differences (P < 0.01). The levels of β-endorphin in model group, needle knife group and round needling group were lower than those in normal group, levels of PGE2 in model group, needle knife group and round needling group were higher than those in normal group, and the differences were statistically significant (P < 0.05 or P < 0.01). The levels of β-endorphin in needle knife group and round needling group were higher than those in model group, levels of PGE2 in needle knife group and round needling group were lower than those in model group, and the differences were highly statistically significant (P < 0.01). Conclusion The needle-knife loosening method is more effective than round needles in improving behavioral scores, and promoting the synthesis of β-endorphin and inhibiting the synthesis of PGE2 may be one of its mechanisms.
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