|
|
|
| The protective effect of Cangai Volatile Oil on upper respiratory tract in the mice of smoking model |
| CHEN Bojun WANG Jinjin CHEN Zonghan YANG Zhongshan XIONG Lei |
| Yunnan University of Traditional Chinese Medicine, Yunnan Province, Kunming 650500, China |
|
|
|
Abstract Objective To investigate the safety of Cangai Volatile Oil （CAVO） by inhaled administration and the protective effect. Methods A total of 60 SPF Kunming mice with half male and half female were selected, and 20 of them were randomly divided into blank group and drug group (CAVO 2%) by random number table method, with 10 mice in each group for study the safety evaluation of CAVO. The other 40 were divided into the passive smoking model group (mode group), the CAVO high (3%), medium (2%) and low (1%) dose groups according to the random number table method, with 10 mice in each group for the study of protective effect of CAVO on upper respiratory tract. The model group were replicated in all groups except the blank group. Light 3 cigarettes in the homemade smoke room, once in the morning and once in the evening, for 20 days in total. The high, medium and low dose CAVO groups were molded and inhaled for 20 days at the same time, once in the morning and once in the evening, each lasting for 30 min. Secretory immunoglo-bulin A (SIgA) content in saliva of mice was determined by enzyme-linked immunosorbent assay on the 21st day of model administration. White blood cell count and percentage of neutrophils in mice were measured by eyeball extraction. The tissue of posterior pharyngeal wall was taken out for observation. Results There was no statistically significant difference in the white blood cell count and percentage of neutrophils, content of SIgA and ocular tissues between the drug group and the blank group (P ＞ 0.05). Compared with the blank group, the content of SIgA in the model group was significantly decreased, and the white blood cell count and the percentage of neutrophils were significantly increased (P ＜ 0.01), and the score of lesion degree in the pathological sections of pharyngeal tissues was increased (P ＜ 0.01). Compared with the model group, the content of SIgA increased and the white blood cell count and percentage of neutrophils decreased in the CAVO high, medium and low dose groups (P ＜ 0.01). The score of lesion degree in pharyngeal histopathological sections decreased in CAVO medium and high dose groups (P ＜ 0.01). Conclusion CAVO inhalation administration can reduce the damage of the upper respiratory tract caused by passive smoking, repair the mucosal barrier of the upper respiratory tract, protect the mucosal immune function of the upper respiratory tract of mice, and has no stimulation to the upper respiratory tract of mice.
|
|
|
|
|
|
[1] 世界卫生组织.关于二手烟雾的10个事实[EB/OL].（2016-12-20）.http://www.who.int／features／factfiles／tobaco／tobacco-facts／zh／index2.html.
[2] Joya X，Manzano C，?譧lvarez AT，et al. Transgenerational exposure to environmental tobacco smoke [J]. Int J Environ Res Public Health，2014，11（7）：7261-7274.
[3] 国家药典委员会.中华人民共和国药典四部通则[M].北京：中国医药科技出版社，2015：203.
[4] 周俐，叶开和，任先达.吸烟致小鼠慢性支气管炎模型的免疫功能变化[J].医学综述，2005，11（10）：954-955.
[5] C.F.A.Culling.组织病理学和组织化学技术手册[M].孔庆雷译.北京：科学出版社，1982：80.
[6] 张喆，胡晶红，姚成芳，等.阿胶、黄明胶对被动吸烟小鼠肺脏Th17／Treg细胞亚群分化及相关细胞因子表达的影响差异[J].中国免疫学杂志，2019，35（1）：35-40.
[7] 郭艳，张浩玲，何伦发，等.室内家居环境对儿童青少年呼吸系统健康影响[J].中国公共卫生，2019，８：1-5.
[8] 曹向可，王维，钱庆增，等.3岁以下婴幼儿喘息的危险因素分析[J].广东医学，2017，38（10）：1548-1551.
[9] Blizzard L，Ponsonby AL，Dwyer T，et al. Parental smoking and infant respiratory infection：how important is not smoking in the same room with the baby? [J]. Am J Public Health，2003，93（3）：482-488.
[10] Cohen NA，Zhang S，Sharp DB，et al. Cigarette smoke condensate inhibits transepithelial chloride transport and ciliary beat frequency [J]. Laryngoscope，2009，119（11）：2269-2274.
[11] Karaman M，Tek A. Deleterious effect of smoking and nasal septal deviation on mucociliary clearance and improvement after septoplasty [J]. Am J Rhinol Allergy，2009， 23（1）：2-7.
[12] Tamashiro E，Xiong G，Anselmo-Lima WT，et al. Cigarette smoke exposure impairs respiratory epithelial ciliogenesis [J]. Am J Rhinol Allergy，2009，23（2）：117-122.
[13] 陈柏君，熊磊.浅论吸入给药[J].云南中医学院学报，2009，32（5）：63-66.
[14] Bugova G，Janickova M，Uhliarova B，et al. The effect of passive smoking on bacterial colonisation of the upper airways and selected laboratory parameters in children [J]. Acta Otorhinolaryngol Ital，2018，38（5）：431-438.
[15] Zhang W，Case S，Bowler RP，et al. Cigarette smoke modulates PGE（2）and host defence against Moraxella catarrhalis infection in human airway epithelial cells [J]. Respirology，2011，16（3）：508-516.
[16] 陈柏君，杨梅，许琰，等.浅议肺主气与黏膜免疫的关系[J].南京中医药大学学报，2014，30（3）：210-212.
[17] 黄秋艳，熊磊，孔淑君，等.苍艾挥发油经鼻吸收的药代动力学及组织分布[J].中国实验方剂学杂志，2016，22（23）：94-97.
[18] 解宇环，莫愁，李刚，等.苍艾挥发油对肺气虚大鼠肺组织病理形态和T细胞亚群的影响[J].时珍国医国药，2015，26（8）：1845-1847.
[19] 张怡，沈旭成，叶兴东.桉树脑与皮肤屏障相关性研究进展[J].皮肤性病诊疗学杂志，2019，26（3）：191-194.
[20] 张季林，魏惠珍，张洁.β-石竹烯生物学功能的研究进展[J].山东医药，2018，58（38）：110-112.
[21] 王磊，李跟旺.广藿香抗菌消炎调节免疫作用的最新研究[J].西部中医药，2018，31（2）：138-140. |
|
|
|
