运脾柔肝方对便秘型肠易激综合征大鼠胃动素、生长抑素影响的实验研究

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Abstract Objective To explore the effect of Yunpi Rougan Recipe on the expression of motilin (MOT) and somatostatin (SS) in serum and colon tissues of rats with constipation type irritable bowel syndrome (IBS-C). Methods Twelve Wistar rats were selected as normal group, IBS-C model was established by ice water gavage in the other 60 rats. After the successful modeling, the rats were divided into IBS-C model group, Mosapride group and low, medium and high dose of traditional Chinese medicine group according to the random number table method, with 12 rats in each group. Normal group and IBS-C model group were given equal volume distilled water, Mosapride group was given 1.35 mg/(kg·d) suspension, low, medium and high dose of traditional Chinese medicine groups was given 12.2, 24.4, 48.8 g/(kg·d) respectively. The expression of MOT and SS in serum and colon tissue of rats were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. Results After molding, compared with normal group, the number of fecal particles in IBS-C model group decreased (P < 0.05). After administration, compared with normal group, the number of fecal particles in IBS-C model group decreased (P < 0.05); compared with the IBS-C model group, the number of fecal particles in Mosapride group and low, medium and high dose of traditional Chinese medicine groups increased (P < 0.05 or P < 0.01). After modeling, compared with normal group, the fecal water content of IBS-C model group decreased (P < 0.01). After administration, compared with normal group, the fecal water content of IBS-C model group decreased (P < 0.05); compared with IBS-C model group, the fecal water content of low, medium and high dose of traditional Chinese medicine groups increased (P < 0.01). Compared with normal group, the intestinal propulsion rate of IBS-C model group was significantly decreased (P < 0.05); compared with IBS-C model group, the intestinal propulsion rate of Mosapride group and low, medium and high dose of traditional Chinese medicine groups was significantly increased (P < 0.05 or P < 0.01). Compared with normal group, the content of serum MOT in IBS-C model group decreased, and SS increased (P < 0.05); compared with IBS-C model group, the content of serum MOT in Mosapride group, middle and high dose of traditional Chinese medicine groups increased, and SS decreased (P < 0.05). Compared with normal group, the MOD value of the colon tissue of IBS-C model group was significantly decreased (P < 0.05), and the MOD value of SS was significantly increased (P < 0.05); compared with the IBS-C model group, the MOD value of mot was significantly decreased (P < 0.05), and the MOD value of SS was significantly decreased (P < 0.05). Conclusion Yunpi Rougan Recipe may regulate gastrointestinal hormones to improve intestinal propulsion rate and defecation by regulating the expression of MOT and SS in rat serum and colon tissues.

Key words： Yunpi Rougan Recipe Constipation type irritable bowel syndrome Motilin Somatostatin

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	LIU Mengru1 QIU Renjing1 TIAN Yaozhou2 LI Hui2
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LIU Mengru1 QIU Renjing1 TIAN Yaozhou2 LI Hui2,3. Experimental study on effect of Yunpi Rougan Recipe on motilin and somatostatin in rats with constipation type irritable bowel syndrome[J]. 中国医药导报, 2020, 17(8): 4-8,16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I8/4

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