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| Effects of Bulbus Fritillariae Cirrhosae medicated serum on bronchial smooth muscle cells and vascular endothelial growth factor |
| LI Houzhong1 LIU Li2 HUANG Wei3 ZHOU Fubo1 ZHAI Fengguo1 YANG Xudong1 ZHANG Yufei4 |
1.Pharmacology Teaching and Research Section, Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157011, China;
2.Department of Recovery, Hongqi Hospital Affiliated to Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157011, China;
3.Academic Administration, Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157011, China;
4.Medical Research Center, Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157011, China |
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Abstract Objective To investigate the effect of Bulbus Fritillariae Cirrhosae medicated serum on the proliferation of the bronchial smooth muscle cells (BSMC), the tube formation and the expressions of vascular endothelial growth factor (VEGF) in BSMC. Methods The murine were sensititized and challenged with ovalbumin (OVA) to establish asthma model and Bulbus Fritillariae Cirrhosae medicated serum was prepared. The BSMC were cultured in vitro and randomly divided into the blank serum group, the low dose group and the high dose group. The proliferations of BSMC, the lengths of the tube formation, the expressions of VEGF and VEGF mRNA were detected. Results Compared with the blank serum group, the proliferations of BSMC, the lengths of the tube formation, the expressions of VEGF and VEGF mRNA of the low dose group and the high dose group were increased significantly (P < 0.05). Conclusion Bulbus Fritillariae Cirrhosae medicated serum can inhibit the proliferations of BSMC and the lengths of the tube formation, and the possible mechanisms may be related to reduce the expressions of VEGF.
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