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| Application effect of high-throughput sequencing in patients with severe pulmonary infection |
| REN Di1 LI Ying1 ZENG Jingjing1 TAN Tingting2 |
1.Department of Critical Care Medicine, the Second People′s Hospital of Shenzhen, Guangdong Province, Shenzhen 518000, China;
2.Department of Nephrology, the Chinese Traditional Medicine Hospital of Shenzhen, Guangdong Province, Shenzhen 518000, China |
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Abstract Objective To investigate the effect of high-throughput sequencing technology in patients with severe pulmonary infection. Methods A retrospective analysis of the clinical data of 87 patients with severe pulmonary infection were admitted to the Department of Critical Medicine, Shenzhen Second People′s Hospital from January to December 2018. Random table method was used to divide 43 cases of next generation sequencing (NGS) group and 44 cases of control group. The pathogen detection rate in the NGS group, the intensity of antibiotic use, the number of days in the intensive care unit (ICU), and the 28-day mortality of two groups were compared. Results There was no significant difference in baseline data of patients between the two groups before treatment (P ＞ 0.05). The detection rate of high-throughput pathogens in the NGS group was significantly higher than that of conventional culture, the intensity of antibiotic use in the NGS group was lower than that in the control group, and the ICU hospital stay was shorter than that in the control group, the differences were statistically significant (P ＜ 0.05). There was no significant difference in the 28 days mortality between the two groups (P ＞ 0.05). Conclusion The application of high-throughput sequencing technology can improve the detection rate of alveolar lavage fluid pathogens in patients with severe pulmonary infection, reduce the intensity of antibiotic use and shorten the length of hospitalization.
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