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| Incidence of chorionic amnitis in patients with premature rupture of fetal membranes and the correlation between NLRP3 expression and Inflammatory cytokines, proteases |
| CAI Shumei1 WANG Yamin1 CHEN Hui2 MENG Yuanyuan1 ZOU Changjun1 LI Yan1▲ ZHANG Jinling1 |
1.Department of Obstetrics, Handan Maternal and Child Health Care Hospital, Hebei Province, Handan 056001, China;
2.Department of Gynecology, Handan Central Hospital, Hebei Province, Handan 056001, China |
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Abstract Objective To investigate the incidence of chorionic amnitis in patients with premature rupture of fetal membranes and the correlation between NLRP3 expression and Inflammatory cytokines, proteases. Methods From March 2017 to February 2019, 138 cases of pregnant women with premature rupture of fetal membrane admitted to Handan Maternal and Child Health Hospital in Hebei Province were selected. The occurrence of chorionic amnitis in pregnant women with premature rupture of fetal membrane was retrospectively analyzed, and the patients were divided into infection group (49 cases) and non-infection group (89 cases). Placenta tissue expression of NLRP3 inflammatory corpuscle, inflammatory cytokines[interleukin 6 (IL-6) and IL-8 and IL-18, tumor necrosis factor-α (TNF-α)], protease[neutrophil elastase(NE), matrix metalloproteinase-2(MMP-2) and matrix MMP-9] levels in serum and amniotic fluid specimens between two groups were compared. Pearson test was used to evaluate the correlation between expression of NLRP3 inflammatory corpuscle and disease-related indicators in patients with premature rupture of membranes and chorionic amnitis. Results Expression level of NLRP3 inflammatory corpuscle in infected group was higher than that in non-infected group (P ＜ 0.05). Levels of IL-6, IL-8, IL-18 and TNF-α in serum and amniotic fluid were higher than those in non-infected group; levels of NE, MMP-2 and MMP-9 were higher than those of non-infected group (P ＜ 0.05). Correlation analysis showed that the expression of NLRP3 inflammasome in placental tissues of premature rupture of membranes patients combined with chorionic amnitis was positively correlated with the levels of IL-6, IL-8, IL-18, TNF-α, NE, MMP-2 and MMP-9(rNLRP3=0.674, 0.718, 0.698, 0.829, 0.759, 0.726, 0.882；rASC = 0.704, 0.783, 0.627, 0.599, 0.771, 0.804, 0.837；rcaspase-1=0.667, 0.572, 0.782, 0.705, 0.812, 0.632, 0.576, all P ＜ 0.05). Conclusion High expression of NLRP3 inflammasome may be one of the important mechanisms of premature rupture of membranes in pregnant women with chorionic amnitis.
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