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| Effect of human luteinizing hormone on clinical outcome during in-vitro fertilization or intracytoplasmic sperm injection-embryo transfer |
| XIA Rong YANG Pan LI Jianjun GUO Haichun ZHANG Jianmei XIAO Yaling |
| Reproductive Center, Changsha Maternal and Child Health Hospital, Hu′nan Province, Changsha 410007, China |
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Abstract Objective To investigate the effect of human luteinizing hormone (LH) on clinical outcome during in-vitro fertilization or intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). Methods Clinical data of patients with ovulation induced through long follicle stage regimen at the Reproductive Center of Changsha Maternal and Child Health Hospital from January 2017 to August 2018 were analyzed retrospectively, 188 cycles. The patients were divided into four groups based on whether the LH preparation was added during the ovulation promotion or not: Group A [human menopausal gonadotropin (HMG) group, 72 cycles]: HMG was included in addition to follicle-stimulating hormone (FSH), during ovulation]. Group B (r-LH group, 34 cycles): r-LH was added in addition to FSH in the later stage. Group C (HMG+r-LH group, 51 cycles): HMG was added during ovulation promotion and r-LH group was added in the later stage (no LH group, P < 0.05). Group D (31 cycles): no LH preparation was added. Only FSH was used to promote ovulation until the HCG injection day. Thereafter, the number of eggs, normal fertilization rate, high quality embryo rate, clinical pregnancy rate and embryo implantation rate were compared. Results No significant differences were observed in age, infertility years, blood LH level, number of oocytes acquired, normal fertilization rate and embryo rate among all groups (P > 0. 05). There was highly statistically significant difference in total dose of gonadotropin (Gn) among all groups (P < 0.01), and the total doses of Gn of group A, B, and C were higher than that of group D, the differences were statistically significant (P < 0.05). There was highly statistically significant difference in usage days of Gn among all groups (P < 0.01), and the usage days of Gn of group B and C were longer than those of group A and D, the differences were statistically significant (P < 0.05). There was no statistically significant difference in the number of oocytes, normal fertilization rate, high quality embryo rate and implantation rate in each group (P > 0.05). There was statistically significant difference in clinical pregnancy rate among four groups (P > 0.05), the clinical pregnancy rate of group B were higher than that of those group A and D, the differences were statistically significant (P < 0.05). Conclusion For the patients with ovulation induced by long regimen in early follicular phase, the addition of LH, especially r-LH, exhibited a tendency to increase the clinical pregnancy rate. However, it still needs to be verified through a study based on larger sample size.
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