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| Effect of Regesi regeneration silicon and Bio-Oss bone meal on proliferation and osteogenic differentiation of osteoblasts |
| JIA Yan1 ZHANG Baorong2 |
1.Oral Medicine School, Weifang Medical College, Shandong Province, Weifang 261053, China；
2.Oral Clinic, Aviation General Hospital, Beijing 100012, China |
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Abstract Objective To observe the growth of mouse osteoblast (MC3T3-E1) on Regesi regeneration silicon and Bio-Oss bone powder, and to analyze the effects of Regesi regeneration silicon materials and Bio-Oss bone powder on mouse osteoblast proliferation and osteogenic differentiation. Methods The experiment was divided into three groups, respectively Regesi regeneration silicon group, Bio-Oss bone powder group and control group. The two materials were placed in 24-well plates, while the control group was blank. Cells were inoculated on 24-well plates in each group, and the cell growth was observed by inverted microscope at 1, 4 days, and 7 days of culture; MTT was used to detect cell proliferation activity at 1, 4 days, and 7 days of culture; OD value of osteoblasts was determined by alkaline phosphatase (ALP) kit at 7 d culture to analyze ALP activity. Rt-PCR method was used to determine Runx2 and Osterix(OSX) gene expression in 3 groups of cells at 7 days of culture. Results Under the microscope, the number of cells in each group increased with time. The proliferation level of cells in Regesi group increased (P ＜ 0.05), and the ALP activity elevated,the expression levels of Runx2 and OSX mRNA increased at 7 days of culture(P ＜ 0.05). The proliferation level of cells in the bio-Oss bone meal group was decreased at 7 days of culture(P ＜ 0.05), and there was no significant difference in the ALP activity, and the expression levels of Runx2 and OSX mRNA (P ＞ 0.05). Conclusion Regesi regenerated silicon can promote the proliferation and differentiation of MC3T3-E1 cells. Bio-Oss bone powder can decrease cell proliferation, while there is no significant difference between osteogenic differentiation ability and osteoblasts alone.
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