DNMT1和DNMT3a在结直肠癌中的表达、临床意义及相关性研究

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Abstract Objective To explore the expression, clinical significance and correlation of DNA methyltransferase-1 (DNMT1) and DNA methyltransferase-3a (DNMT3a) in human colorectal adenocarcinoma tissues. Methods Fifty specimens of colorectal adenocarcinoma tissues (cancer group) and 50 specimens of normal colorectal tissues (normal group) in the First Affiliated Hospital of Hebei North University from February 2017 to February 2018 were selected. The expression levels of DNMT1 mRNA and DNMT3a mRNA were detected by reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR), and the expression levels of DNMT1 and DNMT3a protein were detected by immunohistochemistry technique. The clinical data was collected to analyze the relationship between their expression and clinicopathological characteristics. The correlation was analyzed by Spearman test. Results The qRT-PCR results showed that the expression level of DNMT1 mRNA and DNMT3a mRNA in the cancer group were higher than those in the normal group, the differences were all statistically significant (all P ＜ 0.05). The immunohistochemical staining results showed that the positive rates of DNMT1 and DNMT3a protein in cancer group were higher than those in the normal group, the differences were statistically significant (all P ＜ 0.05). The expression of DNMT1 and DNMT3a protein in cancer group was correlated with the depth of tumor invasion, TNM stage, lymph node metastasis, and liver metastasis (all P ＜ 0.05), which was not correlated with tumor size and degree of differentiation (all P ＞ 0.05). Spearmen correlation analysis showed that there was significant positive correlation between DNMT1 and DNMT3a in cancer tissues (r = 0.455, P ＜ 0.05). Conclusion The abnormal expression of DNMT1 and DNMT3a plays an important role in the occurrence and development of colorectal cancer, they have a synergistic effect, both can be used as potential biological indicators for early diagnosis and treatment monitoring of colorectal cancer.

Key words： DNA methyltransferase-1 DNA methyltransferase-3a Colorectal neoplasms Reverse transcription quantitative real-time polymerase chain reaction Immunohistochemistry Clinical significance

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	GAO Xiaobin1 WU Xueliang1 ZHAO Yifeng1 NIE Shuangfa1 LIANG Feng1 LIU Xiaofei2 DOU Jinshan3 ZHANG Yingchun1

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GAO Xiaobin1 WU Xueliang1 ZHAO Yifeng1 NIE Shuangfa1 LIANG Feng1 LIU Xiaofei2 DOU Jinshan3 ZHANG Yingchun1. Expression and clinical significance of DNMT1 and DNMT3a in colorctal cancer and its correlation study[J]. 中国医药导报, 2020, 17(1): 17-20,37.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I1/17

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