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| Effect of repetitive transcranial magnetic stimulation with different parameters on the expression of brain-derived neurotrophic factor in serum of rats |
| DONG Guoying CHEN Xia ZHOU Xianju WANG Linxiao |
| Laboratory of Neurological Diseases, the Affiliated Changzhou the Second People′s Hospital of Nanjing Medical University, Jiangsu Province, Changzhou 213003, China |
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Abstract Objective To observe the effect of repetitive transcranial magnetic stimulation (rTMS) with different parameters on serum brain-derived neurotrophic factor (BDNF) in rats. Methods A total of 54 Sprague-Dawle rats were divided into the Sham group (n = 18), 1 Hz rTMS group (n = 12), 10 Hz rTMS group (n = 12), and the intermittent theta burst stimulation (iTBS) group (n = 12). According to the stimulation intensity (the resting motor threshold, rMT), each experiment contains of the Sham group, the low-intensity group (80% rMT) and the high-intensity group (120% rMT), six mice in each group. The prefrontal cortex of rats were treated with rTMS in consecutive five days. Enzyme linked immunosorbent assay (ELISA) was used to detect the change of the serum BDNF level of rats. Results The level of BNDF in the low-intensity 1 Hz rTMS group was significantly higher than that in the Sham group, and the difference was statistically significant (P ＜ 0.05), while there was no statistically significant between the high-intensity 1 Hz rTMS group and the Sham group (P ＞ 0.05). The levels of BDNF among the Sham group, the low-intensity 10 Hz rTMS group and the high-intensity 10 Hz rTMS group were no statistically significant (P ＞ 0.05). The level of BNDF in the high-intensity iTBS group was lower than that of the Sham group, and the difference was statistically significant (P < 0.05), while there was no statistically significant between the low-intensity iTBS group and the Sham group (P ＞ 0.05). Conclusion Intensity is a key parameter affecting the effect of rTMS, while the effect of rTMS in different modes on BDNF varies with the intensity.
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