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| Application of ELISA in the detection of human T-lymphocyte virus antibody detected by in blood donors in Dongguan |
| WANG Qing YU Lin CHEN Qingkai ZOU Jiaoli HE Ziyi CHEN Shaobin |
| Departmant of Laboratory, Dongguan Blood Center, Guandong Province, Dongguan 523930, China |
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Abstract Objective To investigate the application of enzyme-linked immunosorbent assay (ELISA) in the detection of human T-lymphocyte virus (HTLV) antibody in blood donors in Dongguan, and to carry out the corresponding test and ensure the blood safety to provide a reference basis. Methods A total of 68 323 plasma samples were collected from March 2016 to January 2017 in the blood donation in Dongguan Guangdong Province. ELISA was used as a screening method for human T-lymphocyte virus antibodies. Positive samples in the screening were supplemented by electrochemiluminescence (ECLIA) and confirmed by Western blotting (WB). Results Twenty-six samples of HTLV antibody-positive samples were screened by ELISA, the positive rate of screening was 0.038%. Nine samples were positive by ECLIA in the 26 samples, 2 samples were positive by WB, and the positive rate was 0.003%, two cases were all from Fujian Province. The positive predictive value of HTLV antibody was 7.69% (2/26). There were no statistically significant differences in the positive screening rates among blood donors of different genders, ages, educational backgrounds, whether they had donated blood for the first time and whether they were registered in Dongguan (P ＞ 0.05). Conclusion The positive rate of screening and confirmation of HTLV antibodies in blood donors in Dongguan is low, and the positive predictive value is not high. It is recommended to use more specific ELISA or ECLIA for screening. When recruiting, the recruitment of Fujian blood donors can be appropriated strengthened.
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