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| Expression and clinical significance of miR-15b in serum of patients with acute cerebral infarction |
| LI Sheng1 PAN Min1 GU Lian2 LI Shenfen1 |
1.Department of Laboratory Medicine, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China;
2.Department of Encephalopathy, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China |
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Abstract Objective To investigate the expression and clinical significance of serum miR-15b in patients with acute cerebral infarction. Methods From January to December 2018, 98 patients with acute cerebral infarction admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine (“our hospital” for short) were selected as the acute cerebral infarction group, during the same period, 98 healthy subjects in our hospital were as the control group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of serum miR-15b in the two groups. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of miR-15b in acute cerebral infarction. The relationship between serum miR-15b expression and the clinicopathological characteristics of acute cerebral infarction was analyzed. Results Serum miR-15b in the two groups was (1.28±0.38) and (2.33±0.72), respectively. The expression of serum miR-15b in acute cerebral infarction group was higher than that in control group (P ＜ 0.05). The area under the ROC curve of miR-15b in diagnosing acute cerebral infarction was 0.817, 95%CI = 0.721-1.438, the sensitivity was 91.06%, and the specificity was 79.35%. There was no significant difference in serum mir-15b expression among patients of different genders and ages (P > 0.05). There were statistically significant differences in serum miR-15b expression in patients with different infarction degrees, infarction volumes and prognosis (P ＜ 0.05). Conclusion MiR-15b is highly expressed in the serum of patients with acute cerebral infarction and may be related to the degree, volume and prognosis of infarction, which is a potential serological marker for the diagnosis of acute cerebral infarction.
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