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| Effect of NF-κB p65 gene silencing on proliferation and apoptosis of rheumatoid arthritis synovial cells |
| LUO Hai'en MAO Xinzhan |
| Department of Orthopedics, the Second Xiangya Hospital of Central South University, Hu'nan Province, Changsha 410011, China |
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Abstract Objective To investigate the effect of NF-κB p65 gene silencing on proliferation and apoptosis of rheumatoid arthritis synovial HFLS-RA cells. Methods NF-κB p65 siRNA was transfected into HFLS-RA cells by cationic liposome LipofectamineTM2000, and the expression of NF-κB p65 mRNA and protein in HFLS-RA cells was detected by RT-PCR and Western blot respectively. The proliferation and apoptosis of HFLS-RA cells were separately detected by MTT and flow cytometry assay. Results Compared with the blank control group and the negative control group, the expression of NF-κB p65 mRNA, protein and cell proliferation rate in the HFLS-RA cells transfected with siRNA was decreased, while the apoptosis rate was increased, and the differences were statistically significant (all P ＜ 0.05). Conclusion NF-κB p65 gene plays an important role in the proliferation and apoptosis of synovial HFLS-RA cells in rheumatoid arthritis, and silencing its expression can inhibit the proliferation of synovial HFLS-RA cells and induce its apoptosis.
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