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| Study on quality standard of Huoling Shengji Granules |
| WU Mei WU Fei ZHANG Jiquan FENG Yi WANG Youjie |
| Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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Abstract Objective To establish the quality standards for Huoling Shengji Granules. Methods The thin-layer chromatography method (TLC) was used for the qualitative research of Astragali Radix, Atractylodis Macrocephalae Rhizoma and Poria, and the quantitative study of icariin from Epimedii Folium and loganin from Corni Fructus was determined by the high performance liquid chromatography (HPLC). The determination of icariin was carried out by Grace Apollo C18 column (4.6 mm×250 mm, 5 μm)， acetonitrile-water (27:73) was used as the mobile phase of the gradient elute, with the detection wavelength of 270 nm and the flow rate of 1.0 mL/min. The determination of loganin was carried out by Pntulips RSZG C18 column (4.6 mm×250 mm, 5 μm), acetonitrile -0.1% phosphate (8.5:91.5) was as the mobile phase of the gradient elute, with the detection wavelength of 240 nm and the flow rate of 1.0 mL/min. Results In the established TLC method, the spots of Astragalus membranaceus, Atractylodes macrocephala and Poria columba were clear, with good resolution and good specificity. The icariin and loganin in the range of 0.0850-6.3720 μg (r = 1.0000), 0.04774-2.86400 μg (r = 1.0000) showed good linear relationship with the peak area. The average recovery rates (n = 9) of the two components were 97.50%, 100.97% respectively. Conclusion The established methods of TLC and HPLC are simple, and the results are accurate and reliable with a good reproducibility. This quality standards are feasible, which can effectively control the quality of Huolingshengji Granules.
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