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| Study on the protective effect of baicalein on neurotoxicity induced by PrP106-126 fragment |
| QIN Sheng1 PENG Jianyu1 BAI Caiying2 ZHENG Shuilan3 LAN Kai1 CAO Nannan1 CHENG Zhaomin1 ZHOU Qiang1 |
1.Department of Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangdong Province, Guangzhou 510120, China; 2.Department of Clinical Laboratory, Guangdong Women and Children Hospital, Guangdong Province, Guangzhou 511400, China;
3.Guangzhou Huiguanglin Technology Limited Company, Guangdong Province, Guangzhou 510032, China |
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Abstract Objective To explore the protective effect of baicalein on neurotoxicity induced by PrP106-126 and its mechanism. Methods SH-SY5Y cells were divided into cell control group, drug control group, PrP106-126 control group, PrPScr control group and baicalein treatment group according to different treatments. Cytopathic method and MTT were used to detect the effect of PrP106-126 on the morphology and activity of SH-SY5Y cells. The MTT assay was used to detect the efficacy of baicalein in inhibiting the neurotoxicity of PrP106-126. The effect of baicalein on apoptosis induced by PrP106-126 polypeptide was detected by TUNEL. Results The morphology of SH-SY5Y cells infected with PrP106-126 was significantly different from that of cell control. It was found that the photorefractive cells became stronger and the aggregation of cells was broken. Compared with PrPScr control group, the inhibition rate of PrP106-126 was significantly higher (P < 0.05). Compared with PrP106-126 control group, the cell increment rate of baicalein group in the treatment and prevention models was increased significantly (P < 0.05). Compared with cell control group, cell apoptotic index (AI) of PrP106-126 group was significantly higher (P < 0.01). Compared with PrP106-126 control group, cell AI of baicalin treated group was decreased significantly (P < 0.05). Conclusion Baicalein has a therapeutic and prophylactic effects on prions in vitro, and its mechanism may be related to apoptosis.
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