血液来源表皮葡萄球菌生物被膜形成能力及agr基因多态性调查

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Abstract Objective To investigate the gene polymorphism of the accessory gene regular (agr) in blood-derived Staphylococcus epidermidis from clinical isolates, and to understand the molecular characteristics and biofilm formation ability. Methods From March 2012 to February 2015, strains of clinical isolated blood source from Sichuan Province People′s Hospital were collected. Staphylococcus epidermidis was further identified and confirmed by molecular biology method after being identified by automatic microbial identification system. The semi-quantitative adhesion test was used to detect biofilm formation ability, the gene polymorphism of agr was analyzed by multiplex PCR. Results During the experiment, 81 strains of Staphylococcus epidermidis were collected and all of them were included in the next identification and typing experiment. Among 81 strains of the epidermis Staphylococcus epidermidis in blood-derived, 51 (62.96%) agr strains were type Ⅰ, 6 (7.41%) agr strains were type Ⅱ, 7 (8.64%) agr strains were type Ⅲ, 17 (20.99%) agr strains were not parting. There were 54 biofilm-positive strains, accounting for 66.67%. Conclusion The agr gene of in blood-derived from clinical isolated is significantly polymorphic. Blood-derived Staphylococcus epidermidis has a strong biofilm formation ability, that is, has a strong pathogenic rate. Clinicians should pay attention to this phenomenon.

Key words： Staphylococcus epidermidis Agr gene Ploymorphism Biofilm Blood-derived

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	HU Honghua YU Hua HUANG Wenfang YANG Yongchang

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HU Honghua YU Hua HUANG Wenfang YANG Yongchang. Investigation on biofilm formation and gene polymorphism of the accessory gene regular (agr) in blood-derived Staphylococcus epidermidis[J]. 中国医药导报, 2019, 16(32): 18-21.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I32/18

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