芪苓益脑汤对阿尔茨海默病小鼠炎症小体NOD样受体蛋白3表达的影响

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Abstract Objective To investigate the effect of Qiling Yinao Decoction (QYD) on the expressions of nod-like receptor protein 3 (NLRP3) in inflammatory microglia of Alzheimer′s disease (AD) mice. Methods According to the random number table method, 24 male APP/PS1 dual-transgenic mice were divided into the QYD experimental group (18.330 g/kg), Donepezil group (0.001 g/kg) and model group, with 8 mice in each group. And 8 mice of the same litter and the same sex were divided into the control group. Model group and control group were given 2 mL/time of normal saline, and gavage treatment for 30 d. Morris water maze was used to test the learning and memory ability of APP/PS1 dual-transgenic mice. Transmission electron microscope was used to observe the changes of microglia cells in the cerebral cortex of mice. Immunohistochemistry and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect the expressions of NLRP3 in microglia cells in cerebral cortex and β-amyloid (Aβ) in hippocampus. Results Compared with the model group, the ability of learning and memory in QYD group and Donepezil group was significantly improved (all P ＜ 0.05). Compared with the model group, the structure of microglia in cerebral cortex in QYD group and Donepezil group was significantly improved. Compared with the model group, the expression of NLRP3 in cerebral cortex and Aβ mRNA in hippocampus of QYD group and Donepezil group were significantly decreased (P ＜ 0.05). Conclusion QYD can improve the learning and memory ability of AD mice by reducing the expression of NLRP3 in microglia cells in the cerebral cortex of AD mice.

Key words： Qiling Yinao Decoction Alzheimer′s disease mice Nod-like receptor protein 3

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	LI Yang1 FEI Hongxin2 ZHANG Xiaojie1
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LI Yang1 FEI Hongxin2 ZHANG Xiaojie1,3. Effect of Qiling Yinao Decoction on the expression of nod-like receptor protein 3 in inflammasome of Alzheimer′s disease mice[J]. 中国医药导报, 2019, 16(32): 14-17,26.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I32/14

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