对比剂肾病大鼠肾脏bax及bcl-2表达及羟苯磺酸钙干预研究

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Abstract Objective To investigate the expression of bax and bcl-2 in kidney of rats with contrast-induced nephropathy (CIN) and the effects of Calcium Dobesilate. Methods Eighty-four rats were divided into sham operation group (group A), model group (group B) and Calcium Dobesilate intervention group (group C) according to random number table, 28 rats in each group. Group A was given saline, group B was injected with INDO, L-NAME and 760 g/L Diatrizoate, while group C was given Calcium Dobesilate for intragastric administration on the basis of group B. After 12, 24, 48, 72 h model establishment, blood urea nitrogen (BUN) and creatinine (Scr) were measured. Nephropathy was observed by HE staining. Renal cell apoptosis was detected by TUNEL. The expression of bax and bcl-2 were detected by immunohistochemistry and Western blot. Results The levels of BUN and Scr in group B and group C were significantly higher than those in group A at 12, 24, 48, 72 h after the establishment of model, while those in group C were significantly lower than those in group B at 12, 24, 48, 72 h after the establishment of model (P < 0.05). Compared with 12 h after modeling, serum BUN and Scr levels in group B and group C were significantly higher at 24, 48, 72 h after modeling (P < 0.05). Compared with 24 h after model establishment, serum Scr level in group B increased significantly at 48 h after model establishment, serum BUN and Scr levels in group C increased significantly at 48 h after model establishment, and serum Scr level in group C decreased significantly at 72 h after model establishment (P < 0.05). Compared with 48 h after modeling, serum BUN and Scr levels in group B and group C were significantly lower at 72 h after modeling (P < 0.05). In group A, the pathological changes were not significant; in group B, extensive tubular epithelial cell vacuolation, partial tubular necrosis and renal interstitial fibrosis appeared 12 h after modeling; in group C, the renal tubular interstitial lesions were significantly improved. There was no significant apoptosis in group A; the apoptosis index in group B was significantly higher than that in group A; the apoptosis index in group C was significantly lower than that in group B, but higher than that in group A (P < 0.05). The expression level of bax in group B was significantly higher than that in group A, bax in group C was significantly lower than that in group B, but higher than that in group A; the expression level of bcl-2 in group B was significantly lower than that in group A, and the expression level of bcl-2 in group C was significantly higher than that in group B, but lower than that in group A (P < 0.05). The relative expression of bax protein in group B was significantly higher than that in group A, and the relative expression of bax protein in group C was significantly lower than that in group B, but higher than that in group A; the relative expression of bcl-2 protein in group B was significantly lower than that in group A, and the relative expression of bcl-2 protein in group C was significantly higher than that in group B, but lower than that in group A (P < 0.05). Conclusion Apoptosis has close relationship with CIN, and Calcium Dobesilate may play a protective role by reducing the expression of bax and increasing the expression of bcl-2.

Key words： Contrast-induced nephropathy Apoptosis bax bcl-2 Calcium Dobesilate

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	LI Wei1
	2 YU Fenfen3 JI Wenxuan3 HE Xiaoyan1 PAN Peiqiang1
	3 SUN Yan4 Huang Junyan3

Cite this article:

LI Wei1,2 YU Fenfen3 JI Wenxuan3 HE Xiaoyan1 PAN Peiqiang1,3 SUN Yan4 Huang Junyan3. Study on the expression of bax and bcl-2 in kidney of rats with contrast-induced nephropathy and the intervention of Calcium Dobesilate[J]. 中国医药导报, 2019, 16(32): 9-13.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I32/9

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