针刺对大鼠酒精戒断焦虑症的治疗效果及与CRH的关系

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Abstract Objective To observe and explore the effect and mechanism of acupuncture for the treatment of alcohol withdrawal anxiety in rats. Methods Fifty-four SPF healthy male SD rats were selected and randomly divided into blank group, model (alcohol withdrawal anxiety model) group, sānyīnjiāo group, zúsānlǐ group, electro-acupuncture two-point (sānyīnjiāo + zúsānlǐ) group, acupuncture two-point (sānyīnjiāo + zúsānlǐ) group, with 9 rats in each group. Except the blank group, all the other groups were given continuous intraperitoneal injection of alcohol for 28 days to prepare the alcohol addiction model, withdrawal of alcohol for 3 days to prepare the alcohol withdrawal anxiety model, and they were respectively given acupuncture at corresponding points during withdrawal of alcohol for 3 days. The elevated plus-maze (EPM) test was used to measure the anxiety behavior. The enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of corticotropin releasing hormone (CRH) in the hippocampus. The real-time fluorescence quantitative PCR was used to detect the expression of CRH mRNA in hippocampus. Results Compared with the blank group, the percentage of number and residence time of the model group staying in the open arms were significantly reduced (P ＜ 0.05), the concentration of CRH in the hippocampus was significantly increased (P ＜ 0.01), the expression of CRH mRNA was significantly increased (P ＜ 0.01); compared with the model group, the percentage of number of entering the open arm and residence time staying in the open arm during EPM test of the electro-acupuncture two-point group were significantly increased (P ＜ 0.05 or P ＜ 0.01), the percentage of number entering the open arm during EPM test of the acupuncture two-point group was significantly increased (P ＜ 0.05), CRH concentrations in hippocampus of the electro-acupuncture two-point group and the acupuncture two-point group were significantly decreased (P ＜ 0.01), and CRH mRNA expression in hippocampus was also significantly decreased (P ＜ 0.05 or P ＜ 0.01). However, the effect of single acupoint group on each monitoring index was not significant (P ＞ 0.05). Conclusion Acupuncture can relieve the anxiety of rats with alcohol withdrawal anxiety, and its anti-anxiety effect may be related to the regulation of CRH expression in the hippocampus of rats.

Key words： Acupuncture Anxiety Alcohol withdrawal Corticotropin releasing hormone

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	XING Yushuang1
	2 ZHENG Kewen2 LIANG Qichao2 WU Yiyan2 ZHAO Yujia2

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XING Yushuang1,2 ZHENG Kewen2 LIANG Qichao2 WU Yiyan2 ZHAO Yujia2. Effect of acupuncture for the treatment of alcohol withdrawal anxiety in rats and its relationship with CRH[J]. 中国医药导报, 2019, 16(26): 12-16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I26/12

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