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| Effect of PI3K and p-Smad7 expression on proliferation of osteosarcoma cells |
| ZHANG Xiaoping YU Zhe XU Yun YAN Kang LIAO Bo ZHOU Yong GAO Tongshuan |
| National Institute of Bone Oncology, Tangdu Hospital, Air Force Military Medical University of PLA, Shaanxi Province, Xi′an 710032, China |
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Abstract Objective To study the effect of PI3K and p-Smad7 expression on the proliferation of osteosarcoma cells. Methods From January 2017 to October 2018, 60 osteosarcoma patients (osteosarcoma tissues) and 60 chondroid tissues (normal tissues) from Department of Orthopedics Tangdu Hospital, Air Force Military Medical University of PLA were selected as subjects for study and immunohistochemical staining. The changes of two kinds of bone tissues and the expression of PI3K and p-Smad7 in different tissues were observed. Osteosarcoma MG-63 cells were treated with PI3K inhibitor CAL-101 (2.5 nm) as experimental group and untreated osteosarcoma MG-63 cells as control group. The proliferation capacity (MTT proliferation experiment), cloning ability (clonogenic experiment) and expression of PI3K and p-Smad7 (detection of qRT-PCR and Western blot) of MG-63 cells in two groups were observed. Results The expressions of PI3K and p-Smad7 in osteosarcoma tissues were significantly higher than those in normal tissues (P < 0.01). After administration of 48 and 72 hours, proliferation capacity of MG-63 cells in experimental group was significantly lower than that in control group (P < 0.05). After administration of 48 hours and 15 days of continuous culture, cloning ability of MG-63 cells in experimental group was significantly lower than that in control group (P < 0.05). After administration of 36 hours, expression of PI3K and p-Smad7 in MG-63 cells in experimental group was significantly lower than that in control group (P < 0.05). Conclusion Abnormal expression of PI3K and p-Smad7 interaction can promote proliferation of osteosarcoma cells.
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