|
|
|
| The manufacture of electrochemical sensor based on multiwalled carbon nanotube-graphene for detection of the rubella virus IgM antibody |
| LIAO Ang1 DENG Qiming1 DONG Linling1 YU Yanfei1 DIAO Qizhi2 DING Qiang3▲ |
1.Department of Clinical Laboratory, Maternal and Child Health Hospital of Yongchuan District, Chongqing 402160, China;
2.Department of Clinical Laboratory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
3.Department of Clinical Laboratory, Coal Occupational Disease Hospital of Chongqing, Chongqing 402160, China |
|
|
|
Abstract Objective To develop a electrochemical sensor based on multiwalled carbon nanotube-graphene for detection of the rubella virus IgM antibody and to evaluate its analytical performance. Methods Oxidized graphene (GS), chitosan (CHI), multi-wall carbon nano-tubes (MWCNT), 1-butyl-3-methylimidazolium hexafluorophosphate (six-[BMIM][PF6]-NH2), gold nanoparticles and thionine biological nanomaterial were used as materials to modify screen printing electrode by layer-by-layer self-assembly and then rubella antigen was immobilized on the assembled electrode surface, afterwards coated by horseradish peroxidase and eventually formed the electrochemical sensor used in this study. The layer-by-layer assembled electrode was scanned by cyclic voltammetry to detect its electrochemical performance. The optimum experimental conditions of the sensor were obtained by optimization experiments. The analytical performance of the sensor was evaluated under the optimum experimental conditions. Results After the bare electrode was assembled with above-mentioned materials, the peak current and conductivity of the electrode gradually increased, and the response current gradually decreased after being coated by antigen and horseradish peroxidase. The optimum pH value and H2O2 concentration were 7.0 and 8.0 mmol/L respectively. The optimum incubation temperature and time were 35.0℃ and 30 minutes respectively. The repeatability precision and inter-day precision of the detection of rubella virus IgM antibody were 3.4% and 4.9% respectively, the linear range was 0.1-300 pg/mL, the detection limit was 40 fg/mL, the recovery rate was 97.5%. And at the same time, the sensor had a good anti-interference performance. Conclusion Under the optimum experimental conditions, the assembled electrochemical sensor for detection of rubella virus has the advantages of higher sensitivity, better precision and accuracy, the rapid and simple operation and also quantifiability compared with the existing methods.
|
|
|
|
|
|
[1] Dontigny L，Arsenault MY，Martel MJ，et al. Rubella in Pregnancy [J]. Sogc Clinical Practice Guidelines，2008，30（2）：152-168.
[2] Zeng L，Ma CX，Xie GM，et al. Multiwalled Carbon Nanotube-Graphene Nanosheet-Chitosan-1-Butyl-3-Methylimidazolim Hexafluorophosphate Nanocom-posites and Gold Nanoparticle-Thionine for Electrochemical Detection of C-ytomegalovirus Phosphoprotein [J]. J Nanosci Nanotechnol，2016，16（7）：6726-6733.
[3] Kavosi B，Salimi A，Hallaj R，et al. A highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/PAMAM dendrimer loaded on MWCNTS/chitosan/ionic liquid nanocomposite [J]. Biosensors Bioeletronics，2014， 52（1）：20-28.
[4] Bhalla V，Carrara S，Sharma P，et al. Gold nanoparticles mediated label-free capacitance detection of cardiac troponinⅠ[J]. Sensors Actuators B，2012，161（6）：761-768.
[5] Yang H，Shan C，Li F，et al. Covalent functionalization of poly-disperse chemically-converted graphene sheets with amine-terminated ionic liquid [J]. Chem Commun，2009， 11（11）：3880-3882.
[6] 杨俊梅，李进香，郭振欣，等.风疹病毒IgG抗体亲和力检测对近期感染诊断的应用价值[J].中华实验和临床病毒学杂志，2014，28（2）：142-144.
[7] Wu S，He Q，Tan C，et al. Graphene-based electrochemical sensors [J]. Small，2013，9（8）：1160-1172.
[8] Karthika P，Rajalakshmi N，Dhathathreyan KS. Phosphor-Doped exfoliated Grapheme for supercapacitor electrodes [J]. J Nanosci Nanotechnol，2013，13（3）：1746-1751.
[9] 曾莉，刁奇志.石墨烯修饰的电化学免疫传感器在肿瘤标志物中的应用[J].检验医学与临床，2012，12（9）：1314.
[10] 严银芳，陈晓，姚学军，等.风疹病毒特异性IgM诊断试剂盒的研制和临床应用[J].湖北医科大学学报，2000， 21（4）：268-269，279.
[11] 谭玉华，于婷，李奕辉，等.时间分辨荧光免疫法风疹病毒IgG抗体定量测定试剂盒的研制与性能评价[J].国际检验医学杂志，2014，35（4）：472-474.
[12] 谭玉华，于婷，陈建起，等.抗风疹病毒IgM抗体生物素亲和素时间分辨荧光免疫分析捕获法的建立与性能评价[J].临床检验杂志，2013，31（12）：901-903. |
|
|
|
