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| Association study of fibrinogen genepolymorphisms and haplotypes in ischemic stroke |
| OU Ningjiang1 DONG Liangzhe1 OU Yanping1 CHEN Xuan2 LI Guohui2 LI Jun1 CAI Yonglin1 TANG Minzhong1 |
1.Department of Clinical Laboratory, Wuzhou Red Cross Hospital, Guangxi Zhuang Autonomous Region, Wuzhou 543002, China;
2.Department of Neurology, Wuzhou Red Cross Hospital, Guangxi Zhuang Autonomous Region, Wuzhou 543002, China |
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Abstract Objective To discuss the relationship between fibrinogen (Fg) gene polymorphism and its haplotype with cerebral infarction. Methods From January 2014 to November 2015, in Wuzhou Red Cross Hospital, 255 patients with cerebral infarction were selected as case group, and at same time, 262 healthy persons were selected as control group. βBcl-1 G/A and αTaq-1 site gene typing were taken by polymerase chain reaction (PCR) -restricted fragment length polymorphism analysis technology, Taqman real-time fluorescence quantification PCR probe method was applied to take 448G/A, -148C/T, -455G/A, -854G/A, rs6050 for gene typing, 1211A/G, 1202C/T were analyzed by nucleotide sequence determination, the establishment and analysis of gene haplotype were made by Haploview software, and finally Clauss method was taken to measure blood-plasma Fg level. Results In 9 Fg genetic locus, the difference of genotype frequency and allele frequency of -455G/A polymorphism between case group and control group was statistical significance (P < 0.01), the differences of genotype frequency and allele frequency of 448G/A, -148C/T, -854G/A nucleotide polymorphism between case group and control group were no statistical significance (P > 0.05). The relative risk of -455A carriers suffering from cerebral infarction was 5.203 times higher than that of non-carriers. Both 1211A/G, 1202C/T were wild types, mutation sites were not found. Haplotype analysis result showed that close linkage was in 3 sites that-455G/A, -148C/T, 448G/A, haplotype formed by it and occurrence of cerebral infarction had significant positive correlation, and haplotype ATA and ATG had statistically significant differences (P < 0.01). Conclusion Fg gene and cerebral infarction occurrence had significant haplotype correlation, Fgβ-455G/A, -148C/T, 448G/A may be the risk factors of people associated with cerebral infarction.
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