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| Protective effects of phycocyanin on myocardium in rats with acute myocardial ischemia reperfusion injuries |
| CAO Mi XU Liqin LIU Qingzhong QI Xushen |
| Department of Pediatrics, Taihe Hospital Affiliated Hospital of Hubei University of Medical, Hubei Province, Shiyan 442000, China |
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Abstract Objective To observe the protective effects of phycocyanin on myocardium in rats with acute myocardial ischemia reperfusion injuries (MIRI), and explore the mechanism. Methods The model of acute myocardial ischemia reperfusion injury was established by ligating the left anterior descending coronary artery of 48 SPF SD rats with 30 min after reperfusion, then they were divided into 3 groups: model control group, phycocyanin group A and group B, another 8 rats were selected as normal control group. Rats in phycocyanin group A and group B were treated with phycocyanin by intragastric administration [100, 400 mg/（kg·d）] for 10 d, rats in model control group and normal control group were treated with the same does of 0.9% sodium chloride solution by intragastric administration. The activity of T-SOD and GSH-PX were detected in the tail venous blood, the content of MDA in myocardium was detected, the expression of iNOS in cardiac myocytes was detected by immunohistochemistry. The expression of cyclin dependent kinase inhibitor P21 and P27 in coronary artery was detected by image analysis. Results Compared with model control group and after modelling, the activity of T-SOD and GSH-PX in group A and group B improved significantly, the content of MDA reduced significantly, the expression of cyclin dependent kinase inhibitor P21 and P27 in coronary artery improved significantly, the expression of iNOS in cardiac myocytes reduced significantly, the differences were statistically significant (P < 0.05). Phycocyanin group A were compared with Phycocyanin group B, the differences were not statistically significant (P > 0.05). Conclusion Phycocyanin can protect myocardium with MIRI by promoting P21 and P27 gene expression, improving T-SOD and GSH-PX enzyme activity, controlling iNOS and lipid peroxidation of ischemic myocardium and reducing the myocardial cell pathological vascular remodeling.
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[1] 胡一冰，林玉琳，赵艳芝，等.国外心肌缺血/再灌注引发的细胞凋亡的治疗研究进展[J].中国医药导报，2013，10（5）：32-34.
[2] Kumar D，Bhaskn M，Alagappan L，et al. Heme oxygenase-1 modulates mesangial cell proliferation by p21 Wafl upregulation [J]. Ren Fail，2010，32（2）：254-258.
[3] Herr I，Debatin KM. Cellular stress response and apoptosis in cancer therapy [J]. Blood，2001，98（9）：2603-2614.
[4] 周玉，王伶俐，黄杨，等.糖尿病大鼠的SOD活性与抗氧化剂治疗对机体抗氧化状态的影响[J].中国实验动物学报，2016,24（4）:422-426
[5] 王超，孙峰，丁晓洁，等.藻蓝蛋白在脑缺血再灌注损伤过程中的抗氧化作用[J].中华老年心脑血管病杂志，2007， 9（5）：345-347.
[6] 夏冬，孙军燕，刘娜娜，等.藻蓝蛋白抗氧化作用及其药理活性研究进展[J].海洋科学，2015，39 （7） ：130-135.
[7] 李袁静，蔡明，陈知水，等.建立小鼠急性心肌缺血再灌注损伤模型手术技巧及TTC 染色方法探讨[J].山东医药，2010，50（40）：42-44.
[8] 王文杰，陈红英，彭吉霞，等.红景天苷对大鼠心肌缺血预处理后血管内皮生长因子表达的影响[J].中国医药导报，2015，12（9）：29-33.
[9] 王程，杨金平，罗炜，等.藻蓝蛋白、铁皮石斛多糖及两者配伍对辐射损伤模型小鼠保护作用的研究[J].中南药学， 2016，4（10）：1032-1036.
[10] 沈卫，马璇，刘琳，等.藻蓝蛋白对2型糖尿病大鼠心肌细胞内iNOS表达影响[J].青岛大学医学院学报，2010， 46（4）：283-285.
[11] 褚现明，冷敏，安毅，等.藻蓝蛋白抑制血管平滑肌细胞过度增殖性管腔狭窄的机制研究[J].中国药理学通报，2012，28（10）：1383-1388.
[12] 褚现明，安毅.易损血液与动脉粥样硬化症关系研究进展[J].心血管病学进展，2009，30（5）：867-871.
[13] Lee MS，Yang T，Dhoot J，et al. Meta-analysis of studies comparing coronary artery bypass grafting with drug-eluting stenting in patients with diabetes mellitus and muhtivessel coronary artery dis-ease [J]. Am J Cardiol，2010， 105（11）：1540-1544.
[14] 褚现明，李冰，杜日映，等.P27 对大鼠胸主动脉球囊损伤后管腔狭窄的作用[J].中国动脉硬化杂志，2006 ，14（5）：417-421.
[15] 陈德.氧化还原信号在心肌缺血再灌注损伤中保护作用机制的研究进展[J].中国循环杂志，2013，28（7）:545.
[16] White BC，Sullivan JM，DeGracia DJ，et al.Brain ischemia and reperfusion：molecular mechanisms of neuronal injury [J]. J Neurol Sci，2000，179（Suppl 1-2）：1-33.
[17] 赵艳景，胡虹，王颖 .藻蓝蛋白生理活性及作用机制研究进展[J].安徽农业科学，2011，39 （11） ：6332-6333.
[18] 韩磊，叶平，李鸣皋，等.阿托伐他汀对老年大鼠心肌过氧化氢、丙二醛及谷胱甘肽过氧化物酶水平的影响[J].解放军医药杂志，2014，26（2）：44-46.
[19] 周站平，陈秀兰，陈超，等.藻胆蛋白脱辅基蛋白对其抗氧化活性的影响[J].海洋科学，2003，27（5）：77-81.
[20] 宋璐非，刘冰，赵勇，等.天然藻蓝蛋白对 SD大鼠的长期毒性研究[J].中国医药导报，2012，9（3）：15-21. |
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