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| Bioinformatics analysis and significance of EgAgB8/3 protein from Echinococcus granulosus in Xinjiang Uygur Autonomous Region |
| MA Haimei1,2 Wulamu·Mamuti1,2 ZHANG Fengbo2,3 PANG Pan2,4 ZHAO Hui2,3 DING Jianbing2,4 |
1.Staff Room of Etiology, Basic Medical College, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
2.State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
3.Department of Clinical Laboratory, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
4.Staff Room of Immunology, Basic Medical College, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China |
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Abstract Objective To analyze the amino acid sequencing of EgAgB8/3 protein from Echinococcus granulosus in Xinjiang Uygur Autonomous Region, understand the characteristics of this protein, predict the epitope of its antigen, so as to provide theoretical foundation for further studying and selecting the best alternative antigen of immunological diagnosis and prevention of echinococcosis. Methods The amino acid sequencing and physicochemical property of EgAgB8/3 proteins were predicted by bioinformatics method, the characteristics and secondary structure of EgAgB8/3 proteins were analyzed by different bioinformatics software, and its antigenic epitope was predicted combined with multiple parameters. Results EgAgB8/3 antigen was polypeptide composed of 75 amino acid residues, the relative molecular mass was 8.58×103, isoelectric point was 8.5; the analysis of protein characteristics showed the secondary structure characteristics of α-helix, β-sheet, β-turn and random coil of EgAgB8/3 proteins, among which, there were 3 better areas of β-turn that could be as reference segment of epitope; comprehensive analysis of three software and multiple parameters predicted that, the epitope of EgAgB8/3 protein antigen was mainly located in 1-7 (FVVVAHA), 6-19 (HADDDDDEVTKTKK) and 32-38 (FQSDPLG). Conclusion The prediction for dominant epitope of three B cell epitopes of EgAgB8/3 protein by bioinformatics method has important significance for further studying the antigenicity of EgAgB8/3 and developing more valuable target for immunological diagnosis and prevention of echinococcosis.
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