STAT1基因敲除提高小鼠对EV71病毒的敏感性研究

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Abstract Objective To analyze the differential expression of immune-associated factors in STAT1 knockout mice, and observe the symptoms and viral load after EV71 virus infection, in order to provide a data base for anti-EV71 therapy. Methods Cytometric bead assay (CBA) was used to quantitative analysis the levels of serum immune-associated factors in STAT1 knockout mice after LPS-induced treatment. The symptom and tissue pathological features were observed and the level of muscle virus RNA was quantitated by real-time PCR in STAT1 knockout mice after EV71 virus infection. Results The levels of multi- immune-associated factors were decreased in STAT1 knockout mice after LPS stimulating (P ＜ 0.01), and pathological damage was aggravated accompanied by the higher viral load in muscle (P ＜ 0.01), also severe symptoms and higher mortality when compared to wide type mice after EV71 infection. Conclusions STAT1 plays an important role in the immune responseafter EV71 infection.Thus, STAT1 gene deletion can improve the sensitivity of mice to EV71 virus.

Key words： Virus infection EV71 STAT1 Inflammatory cytokines

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	LI Guangchao ZHANG Qian WU Jing ZHAO Binbin CHEN Xin QIN Chuan LIU Jiangning▲

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LI Guangchao ZHANG Qian WU Jing ZHAO Binbin CHEN Xin QIN Chuan LIU Jiangning▲. Study of STAT1 knockout mice increasing sensitivity in EV71 infection[J]. 中国医药导报, 2017, 14(15): 14-17,30.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I15/14

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