决明子冻干粉对SD大鼠的长期毒性研究

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Abstract Objective To evaluate the chronic toxicity of Semen Cassiae freeze-dried powder in rats. Methods 480 SD rats were randomly divided into 4 groups (60 male and 60 female per group): control group (pure water), low-dose group (0.5 g/kg BW), mid-dose group (2.2 g/kg BW) and high-dose group (10.0 g/kg BW). The volume of drug for gavaging was 1.0 mL/100 g. animals were administrated with drug by 6 days every week for 52 weeks. Subsequently, the weight and foodintake were examined per week. Meanwhile, hematology, blood biochemistry, organ weight, organ coefficient and histopathology were detected on 13-week, 26- week, 39-week and 52-week, and stopping drug at the end of 26-week, 52-week following by 4 weeks recovery period with killing 20 rats in each group. Results ①The result showed there was no death case in each group. ②The weight of rats in dose groups and control group were increased, and it showed dose-related. ③Compared with the control group, the total bilirubin of male rats in mid-dose and high-dose group were lower after 26 weeks (P < 0.01), after recovery period the TBIL of male rats in high-dose group was still lower (P < 0.01). After 39 weeks treatment, the TBIL in high-dose group lower compared with the control group (P < 0.05 or P < 0.01); The triglyceride of male rats in high-dose group was lower after 52 weeks(P < 0.05) and returned to normal after recovered 4 weeks. On 26 weeks and 39 weeks, there were significant increased in relative kidney weights of male rats in mid-dose group and high-dose group compared with control group (P < 0.05 or P < 0.01). ④The toxic target organ in high-dose group was kidney on 26, 39, 52 weeks, and the toxicity was irreversible, no toxic showed in low-dose group and mid-dose group. Conclusion The no-observed-adverse-effect level for Semen Cassiae freeze-dried powder was 2.2 g/kg BW in rats, and the toxic target organ in high-dose (10.0 g/kg BW) group was kidney.

Key words： Semen Cassiae freeze-dried powder Chronic toxicity No-observed-adverse-effect level Toxic target organ

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	WEI Ruoyao PEI Yanyu SUN Jingjiang GAO Hong

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WEI Ruoyao PEI Yanyu SUN Jingjiang GAO Hong. Study on chronic toxicity of freeze-dried powdered Cassiae Semen in Sprague-Dawley rats[J]. 中国医药导报, 2017, 14(15): 4-9.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I15/4

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