紫外分光光度法测定人乳腺癌细胞内外液中阿司匹林的含量

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Abstract Objective To establish an ultraviolet spectrophotometric method for the determination of Aspirin in the intracellular and extracellular fluid of human breast cancer cell line MDA-MB-231 and to dynamically monitor the content of Aspirin in the intracellular and extracellular fluid. Methods The contents of Aspirin in intracellular and extracellular fluids were determined by ultraviolet spectrophotometry with a wavelength of 276 nm. Results In the range of 30~130 μg/mL, the linear relationship between Aspirin concentration and absorbance in the intracellular and extracellular fluid of human breast cancer cell line MDA-MB-231 were good. The regression equation were Y=0.007X-0.021 (r = 0.9995, n = 5); Y=0.006X+0.061 (r = 0.9995, n = 5); the average recoveries were 96.6% (RSD = 1.0%) and 98.4% (RSD = 0.8%), respectively; intraday precision RSD were 2.3% and 2.4%, daytime precision RSD were 2.2% and 2.3%, respectively. The limit of quantification of both intracellular and extracellular fluids was 20 μg/mL, the limit of detection of intracellular fluid was 10 μg/mL, and the limit of detection of extracellular fluid was 1.25 μg/mL. Conclusion Ultraviolet spectrophotometry can be used to determine the content of Aspirin in the intracellular and extracellular fluid of human breast cancer cell line MDA-MB-231. The method is simple, easy, accurate and reproducible.

Key words： Aspirin Ultraviolet spectrophotometry MDA-MB-231 cells Intracellular fluid Extracellular fluid Determination

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	ZHU Xingmei GAO Chen WANG Chuan WANG Guoquan WANG Bin LIU Jiping

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ZHU Xingmei GAO Chen WANG Chuan WANG Guoquan WANG Bin LIU Jiping. Determination of Aspirin in intracellular and extracellular fluid of human breast cancer cells by ultraviolet spectrophotometry[J]. 中国医药导报, 2019, 16(22): 30-32,57.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I22/30

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