三七叶总皂苷抗老年焦虑症的作用及机制研究

Abstract
Figure/Table
References (20)
Related Citation (7)

Download: PDF (837 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the effect and mechanism of total saponins of Panax notoginseng leaves (tSPNL) on late-life anxiety. Methods Fifty aging male mice (12 months) were reared in group (n = 15, normal control group) or isolation (n = 60) according to the random number table method. After 4 weeks, the isolated rearing animal were then randomly divided into model group (vehicle), Diazepam (BDZ) group, tSPNL 500 group (500 mg/kg tSPNL) and tSPNL 100 group (100 mg/kg tSPNL). All mice were intragastrically administered desired compounds once a day for consecutive 14 days. The anxiety-like behaviors were evaluated by elevated plus maze (EPM), and levels of GABA and BDNF in hippocampus were determined by enzyme linked immunosorbent assay after the behavior test. In another set, aging male mice (n = 135) were rearing isolated for 4 weeks and were then randomly divided into model (vehicle), BDZ group, fluoxetine group, tSPNL 500 group. After administration of desired compounds intragastrically once a day for 14 days, animals underwent intraventricular injection of either BDNF receptor TrkB antagonist K252a or GABAA receptor antagonist Bicuculline (Bic), and the anxiety-like behaviors were measured by EPM 1 hour later. Results Compared with model group, the percentage of open arm time (OT%) increased significantly in tSPNL 500 group (P < 0.01), and the levels of BDNF and GABA also increased in hippocampus significantly (P < 0.05 or P < 0.01). After intraventricular injection of K252a and Bic, OT% in tSPNL 500 group decreased significantly (P < 0.05). Conclusion tSPNL can alleviate the anxiety-like behaviors in aging mice, and the mechanism is involved in BDNF-GABA signaling pathway, which may restore the GABAergic plasticity by increasing BDNF levels, thereby reducing the anxiety susceptibility of aging mice.

Key words： Late-life anxiety Total saponins of Panax notoginseng leaves GABA-BDNF signaling pathway GABAergic plasticity

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	DU Yao1
	2 LI Xin1 ZHANG Guixian1 YUAN Yajin1 ZHOU Ningna1

Cite this article:

DU Yao1,2 LI Xin1 ZHANG Guixian1 YUAN Yajin1 ZHOU Ningna1. Study on the effect and mechanism of total saponins of panax notoginseng on late-life anxiety[J]. 中国医药导报, 2019, 16(22): 7-11.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I22/7

[1] Craske MG，Stein MB，Eley TC，et al. Anxiety disorders [J]. Nat Rev Dis Primers，2017，3：17024.
[2] Blazer，DG. Depression in late life：review and commentary [J]. J Gerontol A Biol Sci Med Sci，2003，58（3）：249-265.
[3] Nuss P. Anxiety disorders and GABA neurotransmission：a disturbance of modulation [J]. Neuropsychiatr Dis Treat，2015，11：165-175.
[4] Duveau V，Laustela S，Barth L，et al. Spatiotemporal specificity of GABAA receptor-mediated regulation of adult hippocampal neurogenesis [J]. Eur J Neurosci，2011，34（3）：362-373.
[5] Nikolaus S，Wittsack HJ，Beu M，et al. GABAergic Contyol of Nigrostriatal and Messolimbic Dopamine in the Rat Brain [J]. Front Behav Neurosci，2018，12：38.
[6] Luscher B，Fuchs T，Kilpatrick CL. GABAA receptor trafficking-mediated plasticity of inhibitory synapses [J]. Neuron，2011，70（3）：385-409.
[7] Porcher C，Hatchett C，Longbottom RE，et al. Positive feedback regulation between gamma-aminobutyric acid type A （GABA（A） receptor signaling and brain-derived neurotrophic factor （BDNF） release in developing neurons [J]. J Biol Chem，2011，286（24）：21 667.
[8] Zhou NN，Zhu GB，Lin Q，et al. GABAergic dysfunction in late-life anxiety：the role of BDNF [J]. European Neuropsychopharmacology，2015，25（2）：S253.
[9] 缪吉，雷涛，杨光显，等.三七皂苷对运动性贫血大鼠海马BDNF表达的影响[J].中国康复，2017，32（6）：484-487.
[10] 张华林，李中，周中流，等.三七叶总皂苷对抑郁大鼠脑内单胺及神经因子的影响[J].现代食品科技，2015（12）：32-41.
[11] Allain H，Bentue FD，Polard E，et al. Postural instability and consequent falls and hip fractures associated with use of hypnotics in the elderly：a comparative review [J]. Drugs Aging，2005，22（9）：749-765.
[12] Nutt DJ. Overview of diagnosis and drug treatments of anxiety disorders [J]. CNS Spectr，2005，10（1）：49-56.
[13] Tanguay Bernard MM，Luc M，Carrier JD，et al. Patterns of benzodiazepines use in primary care adults with anxiety disorders [J]. Heliyon，2018，4（7）：88.
[14] 石安华，孙晓菲，周宁娜.老年焦虑症GABA能神经可塑性障碍及BDNF的潜在治疗作用[J].中华行为学与脑科学杂志，2016.25（6）：572-576.
[15] Shinoda Y，Sadakata T，Nakao K，et al. Calcium-dependent activator protein for secretion 2 （CAPS2） promotes BDNF secretion and is critical for the development of GABAergic interneuron network [J]. Proc Natl Acad Sci U S A，2011，108（1）：373-378.
[16] Zhu G，Sun X，Yang Y，et al. Reduction of BDNF results in GABAergic neuroplasticity dysfunction and contributes to late-life anxiety disorder [J]. Behav Neurosci，2019，133（2）：212-224.
[17] Muller HD，Neder A，Sommer C，et al. Different postischemic protein expression of the GABA（A） receptor alpha2 subunit and the plasticity-associated protein MAP1B after treatment with BDNF versus G-CSF in the rat brain [J]. Restor Neurol Neurosci，2009，27（1）：27-39.
[18] Casarotto PC，Bortoil VC，Correa FM，et al. Panicolytic-like effect of BDNF in the rat dorsal periaqueductal grey matter：the role of 5-HT and GABA [J]. Int J Neuropsychopharmacol，2010，13（5）：573-582.
[19] Xu AH，Yang Y，Sun YX，et al. Exogenous brain-derived neurotrophic factor attenuates cognitive impairment induced by okadaic acid in a rat model of Alzheimer′s disease [J]. Neural Regen Res，2018，13（12）：2173-2181.
[20] Keshavarzi S，Kermanshahi S，Karami L，et al. Protective role of metformin against metham-phetamine induced anxiety，depression，cognition impairment and neurodegeneration in rat：The role of CREB/BDNF and Akt/GSK3 signaling pathways [J]. Neurotoxicology，2019，72：74-84.