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| Regulation of Sophora japonica extract on Th17/Treg cell balance in rheumatoid arthritis rats |
| LUAN Zhongqiu1 XIANG Yue2 LI Qiuhong3 WANG Jikun1 |
1.Department of Nephrology, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Graduate School, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.Library, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China |
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Abstract Objective To investigate the effect of Sophora japonica extract on the balance of helper T cells/regulatory T cells (Th17/Treg) in rheumatoid arthritis rats. Methods Fifty male SD rats were fed adaptively for 7 days. Qualified rats were selected and randomly divided into normal group, model group and Sophora japonica group, with 15 rats in each group. A rat model of type Ⅱ collagen induced arthritis was established. Sophora japonica group was given 300 mg/(kg·d) of Sophora japonica extract by gastric perfusion on the 21st day of model establishment, once a day. After 4 weeks of treatment, serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-10, IL-17, IL-21, IL-23 and IL-27 were measured by ELISA. The ratio of Th17/Treg in peripheral blood was detected by flow cytometry. The pathological changes of ankle joint were observed by pathological section. Results Compared with the model group, the levels of TNF-α, IL-6, IL-17, IL-21 and IL-23 in the serum of rats in Sophora japonica group decreased significantly, the levels of IL-10 and IL-27 increased significantly, and the ratio of Th17/Treg in peripheral blood decreased significantly (P < 0.01). Conclusion Sophora japonica can induce cytopoiesis of Tregs while interfering with the differentiation of Th17 by regulating the related immunocytokines, which improves Th17/Treg imbalance, thus exerting therapeutic effect for rheumatoid arthritis.
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