地肤子总黄酮对二硝基氯苯致大鼠接触性皮炎的抗炎作用

Abstract
Figure/Table
References (22)
Related Citation (15)

Download: PDF (1017 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the anti-inflammatory effect of the total flavonoids of Fructus Kochiae (FFK) on DNBC-induced allergic contact dermatitis (ACD) rats. Methods According to random number table method, 40 half male and half female SPF grade SD rats were randomly divided into 5 groups, with 8 rats in each group, including blank control group, ACD model group, positive control group (Splash Methylprednisolone 2.5 mg/kg), FFK low-dose group (75 mg/kg), FFK high-dose group (150 mg/kg). After ACD model were established by treatment of DNCB on right ear for 10 days, rats were treated with FFK in 10 d and the allergic reaction and swelling degree of ears were observed, the content of IL-6, IL-10 and IFN-γ in serum and the level of NF-κB p65 in ears were determined. Results Compared with the blank control group, the swelling of ears in ACD model group was significantly increased, the content of IL-6, IFN-γ in serum and the level of NF-κB p65 in ears were up-regulated, while the content of IL-10 was down-regulated in serum (P < 0.01). Compared with the ACD model group, the ears swelling degree of rats in FFK low-dose group and FFK high-dose group was significantly decreased, the content of IL-6, IFN-γ in serum and the level of NF-κB p65 in ears were significantly down-regulated, while the content of IL-10 in serum was significantly up-regulated (P < 0.01). Conclusion FFK has anti-inflammatory effect on DNBC-induced ADC rats and its mechanism involved in down-regulate the NF-κB p65.

Key words： Total flavonoids of Fructus Kochiae Inflammation Allergic contact dermatitis NF-κB p65

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CAI Pei WEN Xiaoke OUYANG Bo XIAO Zuoqi

Cite this article:

CAI Pei WEN Xiaoke OUYANG Bo XIAO Zuoqi. Anti-inflammatory effect of the total flavonoids of Fructus Kochiae in DNBC-induced allergic contact dermatitis rats[J]. 中国医药导报, 2019, 16(20): 17-20.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I20/17

[1] 国家药典委员.中华人民共和国药典[S].一部.北京：国家医药科技出版社，2015：123-124.
[2] Matsuda H，Dai Y，Ido Y，et al. Studies on Kochiae Fructus Ⅲ. Antinociceptive and Antiinflammatory Effects of 70% Ethanol Extract and Its Component，Momordin Ic from Dried Fruits of Kochia Scoparia L[J]. Biol Pharm Bull，1997，20（10）：1086-1091.
[3] Matsuda H，Dai Y，Ido Y，et al. Studies on Kochiae Fructus Ⅳ. anti-allergic effects of 70% ethanol extract and its component，momordin Ic from dried fruits of Kochia Scoparia L [J]. Biol Pharm Bull，1997，20（11）：1165-1170.
[4] Shin KM，Kim YH，Park WS，et al. Inhibition of Methanol Extract from the Fruits of Kochia Scoparia on Lipopolysaccharide-Induced Nitric Oxide，Prostagladin E2，and Tumor Necrosis Factor-ALPHA. Production from Murine Macr-ophage RAW 264.7 Cells [J]. Biol Pharm Bull，2004，27（4）：538-543.
[5] Lu C，Zhang HY，Ji J，et al. In vivo anthelmintic activity of Dryopteris crassirhizoma，Kochia scoparia，and Polygala tenuifolia against Dactylogyrus intermedius （Monogenea） in goldfish （Carassius auratus）[J]. Parasitol Res，2012，110（3）：1085-1090.
[6] 王建农，刘青云，姚春海，等.地肤子“杀虫止痒”外用功能规范研究[J].中药材，2012，35（12）：1974-1977.
[7] 李培源，卢汝梅，苏炜，等.地肤子总黄酮含量测定及其抗氧化活性[J].湖北农业科学，2016，55（7）：1802-1804.
[8] 张浩，易华，张欣，等.地肤子黄酮类提取物的抗氧化活性研究[J].化工时刊，2012，26（7）：30-32.
[9] 张竞怡，王文杰，史欢，等.地肤子中总黄酮提取工艺及抗氧化性研究[J].浙江农业科学，2011（6）：1340-1344.
[10] Kim JS，Park YM，Jin BJ，et al. Effect of les on recovery capability of DNCB-induced allergic contact dermatitis in rat [J]. J Life Sci，2011，21（5）：713-719.
[11] Yuan Y，Tong L，Wu S. microRNA and NF-kappa B [J]. Adv Exp Med Biol，2015，887：157-170.
[12] Lei L，Wang W，Xia C，et al. Salmonella Virulence Factor SsrAB Regulated Factor Modulates Inflammatory Responses by Enhancing the Activation of NF-kappa B Signaling Pathway [J]. J Immunol，2016，196（2）：792-802.
[13] Liang J，Wang C，Peng J，et al. Naringin regulates cholesterol homeostasis and inhibits inflammation via modulating NF-kappa B and ERK signalling pathways in vitro [J]. Pharmazie，2016，71（2）：101-108.
[14] 李邻峰.皮炎湿疹类皮肤病诊疗进展[J].继续医学教育，2006，20（23）：28-32.
[15] 王德旭，张炜，苏东宝，等.皮炎湿疹致病因素分析[J].中国麻风皮肤病杂志，2008，24（5）：396-397.
[16] William DJ，Timothy GB，Dirk ME. Anderws′ diseases of the skin，clinical dermatology [M]. 10th ed. Philadelphia：WB Saunders，2006：77-82.
[17] Goriely S，Cavoy R，Goldman M. Interleukin-12 family members and type Ⅰ interferons in Th17-mediated inflammatory disorders [J]. Allergy，2009，64（5）：702-709.
[18] Ding HL，Wang XL，Wang HW，et al. Successful treatment of refractory facial acne using repeat short-cycle ALA-PDT：case study [J]. Photodiagnosis Photodyn Ther，2011， 8（4）：343-346.
[19] Yung A，Stables GI，Fernandez C，et al. Microbiological effect of photodynamic therapy （PDT） in healthy volunteers：a comparative study using methyl aminolaevulinate and hexylaminolaevulinate cream [J]. Clin and Exp Dermatol，2007，32（6）：716-721.
[20] Wang C，Yuan J，Wu HX. Total glucosides of paeony inhibit the inflammatory responses of mice with allergic contact dermatitis by restoring the balanced secretion of pro-/anti-inflammatory cytokines [J]. Int Immunopharmacol，2015， 24（2）：325-334.
[21] Huang WY，Wang J，Liu YM. Inhibitory effect of Malvidin on TNF-α-induced inflammatory response in endothelial cells [J]. Eur J Pharmacol，2014，723：67-72.
[22] Martin SF，Esser PR，Weber FC，et al. Mechanisms of chemical-induced innate immunity in allergic contact dermatitis [J]. Allergy，2011，66（9）：1152-1163.