玉郎伞查尔酮对大鼠心肌缺血再灌注损伤后炎性反应的影响

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Abstract Objective To observe the effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone (YLSC) on inflammatory responses in myocardial ischemia reperfusion injury in rats. Methods Sixty 12-month-old SPF Sprague-Dawley (SD) rats were randomly divided into sham group (equal volume of normal saline), model group (equal volume of normal saline), positive control group (Puerarin Injection, 12.00 mg/kg) and YLSC low, medium and high-dose groups (2.50, 5.00, 10.00 mg/kg) via random number table method. The anterior descending coronary artery was ligated for 1 h then reperfused for 3 h to establish the ischemia reperfusion (I/R) model. Rats in sham group were applied stitches under the coronary artery without ligation. Levels of serum troponin I (cTnI), creatine kinase-MB (CK-MB), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were examined by enzyme-linked immunosorbent assay. The infarct sizes were measured by evans blue and 2, 3, 5-triphenyltetrazolium chloride staining. Protein levels of high mobility group box-1(HMGB1), toll-like receptors 4 (TLR4) and nuclear factor-kappa B (NF-κB) in myocardiums were evaluated using Western blot. Results Compared with model group, YLSC reduced the leakage of myocardial enzyme CK-MB and cTnI (P < 0.05 or P < 0.01), decreased myocardial infarction size (P < 0.05 or P < 0.01), lower the release of serum inflammatory cytokines such as IL-6, IL-1β and TNF-α (P < 0.05 or P < 0.01), and inhibited the protein expression of myocardial HMGB1, TLR4, NF-κB (P < 0.05 or P < 0.01). Conclusion YLSC can reduce ischemia-reperfusion injury in rats, the myocardial protective effect may be related to reducing of inflammatory reaction, inhibiting the expression of myocardial related protein such as HMGB1, TLR4 and NF-κB.

Key words： 17-methoxyl-7-hydroxyl-benzofuran chalcone Ischemia and reperfusion injury High mobility group box-1 Inflammatory responses

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	QIN Feizhang1 QIN Qiuhua2

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QIN Feizhang1 QIN Qiuhua2. Effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone on inflammatory responses in myocardial ischemia reperfusion injury in rats[J]. 中国医药导报, 2019, 16(20): 13-16,33.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I20/13

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