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| Effect of pigment epithelium derived factor on ultra structure of acute optic nerve injury in rats |
| ZHAO Junbo1 WANG Bin2▲ YAN Xiaoxiao1 CUI Cui1 |
1.Department of Ophtalmology, Central Hospital of Handan, Hebei Province, Handan 056002, China;
2.Forth Department of Neurology, Central Hospital of Handan, Hebei Province, Handan 056002, China |
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Abstract Objective To investigate the protective effect of pigment epithelium derived factor (PEDF) on mitochondria axon ultra structure of retinal ganglion cells (RGCs) in rats after acute optic nerve injury. Methods 60 SD rats were randomly divided into 3 groups: blank group, model group and experiment group, each group included 20 rats. The blank group was not given any disposal, rats in model group and experiment group were treated with forceps clamping left eye optic nerve to cause incomplete injury of optic nerve, the right eye was not given any disposal. The model group and experiment group, the left eyes models successfully made immediately, at 1 week and 2 weeks, the rats was injected balanced salt solution 5 μL and PEDF 1 μg into vitreous cavity respectively. Rats of three groups were harvested at the third week, 10 rats left eyeball were taken out to make specimens in each group, changes of retinal ultra structure was observed by the electron microscopy; the remaining rats were prepared to make 5 μm retinal slices, the retinal ganglion cells were counted by routine HE staining. Results Mitochondria edema, cytoplasm condensed axon swelling existed in model group and experiment group, compared with the blank group by observing with the electron microscopy. Compared with model group, the degree of cytoplasm condensed axon swelling was lesser, and structure was more completely in the experiment group. HE staining showed ganglion cells in the experiment group and model group were less (P < 0.05), cell morphology of ganglion cells were better and the number of RGCs were more in experiment group. The number of ganglion cell counts in model group and experiment group decreased by 73.3% and 53.3% respectively, the difference was statistically significant (P < 0.05). Conclusion Incomplete injury of optic nerve can cause obvious damage to the ultra structure of the optic nerve, and PEDF can relieve the damage of the ultra structure of the optic nerve., also can weaken the apoptosis of RGCs.
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[1] Tombran-Tink J ， Johnson LV . Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells [J]. Invest Ophthalmol Vis Sci，1989，30（8）：1700-1707.
[2] Steele FR，Chader GJ，Johnson LV，et al. Pigment epithelium-derived factor： neurotrophic activity and identification as a member of the serine protease inhibitor gene family [J]. Proc Natl Acad Sci USA，1993，90（4）：1526-1530.
[3] Ogata N，Wang L，Jo N，et al. Pigment epithelium derived factor as a neuroprotective agent against ischemic retinal injury [J]. Curr Eye Res，2001，22（4）：245-252.
[4] 刁金美，冯国栋，游思维，等.用于中枢神经系统Waller变性研究的新型大鼠视神经夹伤模型[J].神经解剖学杂志，2008，24（3）：239-244.
[5] 江新利，赵平，刘岩.PEDF在眼部疾病中的研究进展[J].眼科新进展，2013，5（5）：485-488.
[6] 李锐，盛文利.色素上皮源性因子-神经营养和保护因子[J].国外医学神经病学神经外科分册，2005，32（3）：289-292.
[7] Houenou LJ，D'CostaAP，Li L，et al. Pigmentepithelium-derivedfactor promotes the survival and differentiation of developing spinalmotor neurons [J]. J Comp Neurol，1999， 412（3）：506-514.
[8] CaoW，Tombran-Tink J，EliasR，et al. In vivo protection ofpho-toreceptors from light damage by pigment epithelium-derived factor [J]. Invest Ophthalmol Vis Sci，2001，42（7）：1646-1652.
[9] 胡鹭萍，徐国兴.色素上皮衍生因子在视网膜疾病中的应用研究进展[J].海峡科学，2007（3）：73-78.
[10] Stellmach，V，Crawford，SE，Zhou，W，et al. Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor [J]. Proc Natl Acad Sci USA，2001，98（5）：2593-2597.
[11] Valnickova Z，Petersen SV，Nielsen SB，et al. Heparin binding induces a conformational change in pigment epithelium-derived factor [J]. Biol Chem，2007，282（9）：6661-6667.
[12] 李锐，盛文利.色素上皮源性因子-一种新的神经营养和保护因子[J].中山大学研究生学刊，2004，25（4）：21-27.
[13] Tombran-Tink J，Barnstable CJ. PEDF： amultifaceted neu?鄄rotrophicfactor [J]. Nat Rev Neurosci，2003，4（8）：628-636.
[14] Yabe T，Wilson D，Schwartz JP. N F-kappa B activation is required for the neuroprotective effects of pigment epithelium-derived factor（PEDF）on cerebellar granule neurons [J]. J Biol Chem，2001，276（46）：43313-43319.
[15] 王亚娜，高莎，沈玺.色素上皮衍生因子对氧诱导视网膜神经节细胞凋亡及Bcl-2表达的影响[J].眼科新进展，2014，34（6）：501-505.
[16] Tan KO，Fu N Y，Sukumaran SK，et al. MAP-1 is a mitochondrial effector of Bax [J]. Proc Natl A cad Sci USA，2005，102（41）：14623-14628.
[17] Tsao YP，Ho TC，Chen SL，et al. Pigment epithelium-derived factor inhibits oxidative stress-induced cell death by activation of extracellular signal-regulated kinases in cultured retinal pigment epithelial cells [J]. Life Sci，2006， 79（6）：545-550.
[18] 李瑞霞，陈海冰.色素上皮衍生因子与氧化应激[J].医学综述，2012，18（13）：1968-1970.
[19] Amano S，Yamagishi S，Inagaki Y，et al. Pigmentepithelium-derived factor inhibits oxidative stress-induced apoptosis and dysfunction of cultured retinal pericytes [J]. Microvasc Res，2005，69（1/2）：45-55.
[20] Kinnally KW，Peixoto PM，Ｒyu SY，et al. Is mPTP the gatekeeper for necrosis，apoptosis or both? [J]. Biochim Biophys Acta，2011，1813（4）：616-622. |
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