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| The relationship between the expression of brain amyloid beta protein along with neural behavior in rats with hypercholesterolemia level |
| LIANG Qian1 TANG Wei2 LIU Wenbo1 WANG Wei1 |
1.Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, Liaoning Province, Dalian 116001, China;
2.Department of Neurology, Affiliated Xinhua Hospital of Dalian University, Liaoning Province, Dalian 116021,China |
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Abstract Objective To research the relationship between the expression of brain amyloid beta (Aβ) protein along with neural behavior in rats with hypercholesterolemia level, in order to clarify the preventive effect of simvastatin on Alzheimer's disease. Methods The 32 Wistar rats were divided into normal group and high cholesterol group according to random number table, with 16 rats in each group. After two weeks, the rats in the normal group were randomly divided into normal drug group and normal placebo group, with 8 rats in each group; and the rats in the high cholesterol group were randomly divided into high fat drug group and high fat placebo group, with 8 rats in each group. And then simvastatin were filled into the stomach of the 16 drug group rats, the same volume placebo (distilled water) were filled into the stomach of placebo group. Immunohistochemical method was used to determine the expression of Aβ protein in brain, Morris water maze was used to test the cognitive and memory abilities in rats. Results Aβ1-42 protein expression of the prefrontal cortex in the high fat drug group was less than that of high fat placebo group (P < 0.05); and there was no significant difference in the high fat drug group, normal placebo group and normal drug group (P > 0.05). In the high fat drug group, the escape latency was decreased than that of high fat placebo group (P < 0.05), times of crossing platform was increased (P < 0.05); and there was no significant difference in the high fat drug group, normal placebo group and normal drug group (P > 0.05). Conclusion Simvastatin has some effects on the expression of Aβ1-42 protein in prefrontal cortex of hypercholesterolemic rats, and accompanied by neurobehavioral changes in rats.
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