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| Study on chemical constituents and their inhibition of anthropogenic colon cancer cells bioactivity from ethnic medicine Pteris dispar |
| SUN Dan ZHANG Yan SUN Qiang PENG Yubo WANG Lei |
| Jiamusi College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China |
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Abstract Objective To study the inhibition of anthropogenic colon cancer cells activity constituents of Pteris dispar. Methods The medicinal materials were extracted by 95% ETOH. Compounds were obtained by kinds of chromatographies including polyamide, Sephadex LH-20 and pre-HPLC and the structures identified by NMR. In vitro antitumor activity was tested by SRB methods. Results 5 compounds were obtained from the plant and their structures were established as geopyxin A (1), geopyxin C (2), ent-11β-acetoxykaur-16-en-18-ol (3), 7β, 16α, 17-trihydroxy-ent-kauran-19-oic acid (4) and neolaxiflorin K (5). Compounds 1, 2 and 5 showed strong in vitro antitumor activity against HCT-116, with IC50 values of 1.20, 2.45 and 3.26 μmol/L respectively. Conclusion Compounds 1-5 are obtained from Pteris dispar for the first time.
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