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| High performance liquid chromatography determination of fucoxanthin content in Sargassum thunbergii of the East China Sea |
| GAO Jie1 WO Lianqun1 WANG Yinyu2 LI Chengping2，3 |
1.Zhejiang Pharmaceutical College, Zhejiang Province, Ningbo 315100, China;
2.College of Pharmaceutical Science, Zhejiang University of Technology, Zhejiang Province, Hangzhou 310032, China;
3.College of Biology and Environment Engineering, Zhejiang Shuren University, Zhejiang Province, Hangzhou 310015, China |
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Abstract Objective To establish an high performance liquid chromatography method for the determination of fucoxanthin content in Sargassum thunbergii of the East China Sea. Methods The samples (20 L) were separated on a Sepax Welch C18 column (4.6 mm×250.0 mm, 5 μm) with a gradient elution of ethyl acetate and 90% aqueous methanol at a flow rate of 1.0 mL/min and detected at 450 nm. The column temperature was kept at room temperature. Results A good linearity was obtained in the test range of 2.7-43.2 μg/mL (r = 0.9997). The limit of detection (3S/N) was 0.027 μg/mL and the limit of quantitation (10S/N) was 0.086 μg/mL. The average recoveries of fucoxanthin were between 99.6% and 105.0%, with a RSD of 1.78% (n = 9). Conclusion The method is simple, sensitive, accurate, and can be used for the quality control of fucoxanthin.
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