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| Study on antipyretic mechanism of Qingkailing Soft Capsules based on metabonomics |
| ZHENG Rui1,2 DU Shouying1,3 LIU Yu2 GUO Rui4 |
1.Traditional Chinese Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
2.Laboratory of Traditional Chinese Medicine Preparation, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;
3.Traditional Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China;
4.Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100029, China |
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Abstract Objective To evaluate the antipyretic effect of Qingkailing Soft Capsules based on metabonomics technology and to explore its related mechanism. Methods A total of 18 SD male rats were randomly divided into control group, model group and administration group, with 6 rats in each group. Model group and administration group were feverished by dry yeast. Rats in the administration group were given 0.5% CMC-Na suspension of Qingkailing Soft Capsules with baicalin as 12.5 mg/kg and model group and blank group were intragastrically administered with 0.5% CMC-Na at the same volume. The metabolic profiles of rat plasma were analyzed under positive and negative ion modes by ultra-performance liquid chromatography-inear ion trap/orbitrap high resolution mass spectrometry (UPLC-LTQ/Orbitrap-MS) metabolomics technique. Through the multivariate statistical analysis method, the data of each group were compared and analyzed, and the potential biomarkers related to fever in plasma were searched. The reverse regulation of Qingkailing Soft Capsules on these endogenous components was explored, and the possible metabolic pathways were inferred. Results On the principal component analysis (PCA) chart, the plasma metabolite profiles of the control group, model group and administration group were separated obviously. Eight potential plasma biomarkers were found and identified, which were lactic acid, nicotinic acid, 3-furoic acid, succinic acid, phosphocholine, arachidonic acid, LysoPE [0∶0/22∶4 (7Z, 10Z, 13Z, 16Z)] and oleic acid. Compared with the control group, the contents of lactic acid (P < 0.01), nicotinic acid (P < 0.01), 3-furoic acid (P < 0.05), succinic acid (P < 0.01) were increased significantly, while the contents of phosphocholine (P < 0.05), arachidonic acid (P < 0.01), LysoPE [0∶0/22∶4 (7Z, 10Z, 13Z, 16Z)] (P < 0.01), oleic acid (P < 0.05) were decreased significantly, which were in the model group. The contents of eight biomarkers in plasma of rats in the administration group were significantly reversed (P < 0.05 or P < 0.01). Conclusion Metabonomics can be used to evaluate the efficacy of Qingkailing Soft Capsules, which may play an antipyretic role by regulating energy metabolism and phospholipid metabolism.
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