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| Intervention effect of Wumoyin Decoction on functional dyspepsia rats of spleen deficiency and Qi stagnation syndrome |
| GAN Guoxing1 WU Xiaofang2 FENG Weixun1 KUANG Weihong3 LI Jingpin4 XIE Baopin5 ZHANG Yizhi1 |
1.Department of Pharmacy, Qingyuan Hospital of TCM, Guangdong Province, Qingyuan 511500, China;
2.School of Nursing, Qingyuan Polytechnic, Guangdong Province, Qingyuan 511510, China;
3.the First Affiliated Hospital of Guangzhou University of TCM, Guangdong Province, Guangzhou 510006, China;
4.College of Pharmacy, Central South University, Hu′nan Province, Changsha 410013, China;
5.Key Laboratory, Prevention and Treatment of Cardiovascular and Cerebrovascular Disease of Ministry of Education of Gannan Medical University, Jiangxi Province, Ganzhou 341000, China |
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Abstract Objective To study the effect of Wumoyin Decoction on FD rats of spleen deficiency and Qi stagnation syndrome. Methods Ten rats were randomly selected as normal group from 70 female SD rats, and other rats were modeled by "chronic restraint + swimming + irregular eating" compound factor method. 48 modeled rats were randomly divided into model group, Domperidone group, Chaishao Liujunzi Decoction group, Wumoyin Decoction high, middle and low dose group, 8 rats in each group. Besides normal group and model group, the other groups of rats were given the corresponding drug intervention for 14 days. The food intake and body weight were recorded, the small intestine propulsion rate was determined, the expression of iNOS, SP, 5-HT in antral tissue and hypothalamus were detected. Results Compared with model group, the food intake of rats in normal group and administration groups were increased significantly on the 5th, 10th and 15th day of administration (P ＜ 0.05 or P ＜ 0.01). Compared with model group, body mass of rats in normal group and Domperidone group were increased significantly on the 5th day of administration (P ＜ 0.05 or P ＜ 0.01). Compared with model group, body mass of rats in normal group and administration groups were increased significantly on the 10th and 15th day of administration (P ＜ 0.05 or P ＜ 0.01). Compared with model group, the propulsive rate of small intestine in normal group and administration groups were increased significantly (P ＜ 0.01). The expression of iNOS in gastric antrum tissue of rats in administration groups and normal groups were significantly down-regulated compared with model group (P ＜ 0.05). Compared with model group, SP expression in gastric antrum tissue of Domperidone group and Wumoyin low dose group rats were significantly deed (P ＜ 0.01). Compared with the model group, the expression of 5-HT in gastric antrum tissue of rats in high dose group of Wumoyin was up-regulated (P ＜ 0.05). Compared with model group, the expression of iNOS in hypothalamus of normal group, Domperidone group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group were significantly increased (P ＜ 0.05 or P ＜ 0.01). Compared with model group, the expression of SP in hypothalamus of normal group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group rats was significantly increased (P ＜ 0.05 or P ＜ 0.01). Conclusion Wumoyin Decoction has certain curative effect on FD rats of spleen deficiency and Qi stagnation syndrome, regulating the expression of iNOS, SP and 5-HT in the brain-gut axis may be one of its mechanisms.
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