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| Simultaneous determination of seven C21 steroidal saponins in Cortex periplocae extract by quantitative analysis of multi-components by single-marker |
| LI Ruixue1 WU Fei1,2,3 ZHANG Jiquan1,2 FENG Yi1,2 |
1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
2.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Zhangjiang Shanghai, Shanghai 201203, China; 3.College of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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Abstract Objective To establish a high performance liquid chromatography method for quantitative analysis of seven C21 steroidal saponins in Cortex periplocae extract by quantitative analysis of multi-components by single-marker. Methods The high performance liquid chromatography analysis was performed on a Capcell Pak C18 column with the detection wavelength at 263 nm. The mobile phase was water containing 0.2% formic acid and acetonitrile in the gradient mode. Periploside C (PSA) was used as the internal marker to establish relative correction factors between PSA and periploside K (PSK), periploside R (WJ53), periploside E (WJ521), periploside D (PSD), periploside Q (WJ33), periploside O (WJ32), and the relative correction factors would be used in the calculation of their contents. The result of quantitative analysis of multi-components by single-marker was compared with external standard method, so as to verify the accuracy of quantitative analysis of multi-components by single-marker. Results The relative correction factors between PSA and PSK, WJ53, WJ521, PSD, WJ33, WJ32 was 0.867, 0.867, 0.847, 0.994, 0.922 and 1.029, respectively. The content values calculated by correction factors and measured values of external standard method did not show significant differences. Conclusion The established quantitative analysis of multi-components by single-marker method is feasible, accurate, simple and can be used as a method for the determination of multi-component indexes in the extract of Cortex periplocae.
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[1] 王欣，覃瑶，王德江，等.一测多评法在中药质量控制中的应用进展[J].中成药，2016，38（2）：395-402.
[2] 徐文芬，杨雯，何顺志，等.一测多评法测定淫羊藿中淫羊藿苷和朝藿定A，B，C[J].中草药，2016，47（1）：130-137.
[3] 杨艳，刘云华，黄志芳，等.一测多评法测定川芎，当归中洋川芎内酯A和藁本内酯的含量[J].中国实验方剂学杂志，2015，21（3）：58-62.
[4] 梅国荣，刘飞，王福，等.一测多评法测定决明子中橙黄决明素，大黄素，大黄酚和大黄素甲醚[J].中草药，2016（8）：1392-1396.
[5] 黄山君，杨琪伟，石燕红，等.一测多评法测定白芍中芍药苷与芍药内酯苷的含量[J].中国中药杂志，2011，36（6）：780-783.
[6] 崔雨晴，张继全，吴飞，等.一测多评法测定假马齿苋中间体中的5种三萜皂苷类成分含量[J].上海中医药杂志，2016，50（7）：88-93.
[7] 李瑞雪，张继全，吴飞，等.香加皮有效成分提取工艺和检测方法的研究进展[J].中国医药导报，2016，13（18）：37-41.
[8] Zhu YN，Zhao WM，Yang YF，et al. Periplocoside E，an effective compound from Periploca sepium Bge，inhibited T cell activation in vitro and in vivo [J]. J Pharmacol Exp Ther，2006，316（2）：662-669.
[9] Wan J，Zhu YN，Feng JQ，et al. Periplocoside A，a pregnane glycoside from Periploca sepium Bge，prevents concanavalin A-induced mice hepatitis through inhibiting NKT-derived inflammatory cytokine productions [J]. Int Immunopharmacol，2008，8（9）：1248-1256.
[10] Zhang J，Ni J，Chen ZH，et al. Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells [J]. Acta Pharmacologica Sinica，2009，30（8）：1144-1152.
[11] Wang LY，Chen ZH，Zhou Y，et al. Structural revision of periplocosides and periperoxides，natural immunosuppressive agents from the genus Periploca [J]. Phytochemistry，2011，72（17）：2230-2236.
[12] Yu B，Sun J，Yang X. Assembly of naturally occurring glycosides，evolved tactics，and glycosylation methods [J]. Acc Chem Res，2012，45（8）：1227-1236.
[13] Zhang X，Zhou Y，Zuo J，et al. Total synthesis of periploside A，a unique pregnane hexasaccharide with potent immunosuppressive effects [J]. Nat Commun，2015，6：5879.
[14] 赵祎武，仲艳，徐振秋，等.高效液相色谱法测定中药香加皮中7种孕甾烷糖苷类成分的含量[J].世界科学技术中医药现代化，2015，17（1）：138-141.
[15] 佟玲，谭晓杰，林景瑞，等.RP-HPLC法测定香加皮中异香草醛、杠柳毒苷和4甲氧基水杨醛的含量[J].药物分析杂质，2009，29（6）：961-963. |
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