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| Effects of aerobic swimming on the expression of acid-sensing ion channel of hippocampus in aging rats |
| LUO Jie1 WANG Jingqiong1 XIE Li′na2 ZHOU Shouhong3 |
1.College of Physical Education, Hu′nan First Normal University, Hu′nan Province, Changsha 410205, China;
2.College of Physical Education, Hu′nan International Economics University, Hu′nan Province, Changsha 410205, China;
3.Department of Physiology, School of Medicine, University of South China, Hu′nan Province, Hengyang 421001, China |
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Abstract Objective To observe the effects of aerobic swimming exercise on the expression of acid-sensing ion channel (ASIC) in aging rats. Methods Thirty-six male specific pathogen free (SPF) Sprague-Dawley (S-D) rats were divided into control group (C group), aging model group (AM group) and aging swimming training group (AST group) by random number table method, with 12 rats in each group. The rats in AM group were administrated with D-galactose [150 mg/(kg·d)] by intraperitoneal injection for 12 weeks. After treatment with D-galactose [150 mg/(kg·d)] by intraperitoneal injection, the rats in AST group were taken 60 minutes of aerobic swimming exercise every day for 12 weeks. The rats in C group were administrated the same amount of saline by intraperitoneal injection. The spatial learning and memory ability of rats were tested by Morris water maze test. Expression of ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4 in hippocampus of rats were measured by Western blot. Results Compared with C group, the time of escape latency and the distance of reaching the platform were significantly increased, the number and time to across platform were significantly decreased, expression of ASIC1a and ASIC1b in hippocampus was significantly up-regulated and expression of ASIC4 was significantly down-regulated in AM group, the differences were statistically significant (P ＜ 0.05). Compared with the AM group, the time of escape latency and the distance to reach the platform were significantly decreased, the number and time to across platform were significantly increased, expression of ASIC1a and ASIC1b in hippocampus was significantly down-regulated and expression of ASIC4 was significantly up-regulated in AST group, the differences were statistically significant (P ＜ 0.05). Conclusion The aerobic swimming exercise improves the learning and memory in aging rats, of which, the mechanism may be related with decrease of expression of ASIC1a and ASIC1b and increase of expression of ASIC4 in hippocampus.
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