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| The protective effects of amniotic mesenchymal stem cells for rats early myocardial injury induced by severe burns |
| MOU Huan1 LIU Yang2▲ LIU Jian3 ZHOU Xinli1 QIANG Tinghui1 DU Xingguo1 DING Chen1 XUE Chao1 |
1.The First Department of Orthopaedic Surgery, Hanzhong Central Hospital, Shaanxi Province, Hanzhong 723000, China;
2.Burn Center of People's Liberation Army, the First Affiliated Hospital of the Fourth Military Medical University, Shaanxi Province, Xi'an 710032, China;
3.Department of Neurosurgery, the First Affiliated Hospital of the Fourth Military Medical University, Shaanxi Province, Xi'an 710032, China |
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Abstract Objective To explore the effects and related mechanism of amniotic mesenchymal stem cells (AMSCs) for rats myocardial injury induced by severe burns. Methods The AMSCs were obtained by the method of collagenase-pancreatic enzyme digestion. Biological markers were assayed with flow cytometry. The activity of AMSCs was identified using adipogenic and osteoplastic differentiation. 24 healthy male SD rats were randomly divided into sham group, burn group and AMSCs group. The dorsal skin of rats in burn group and AMSCs group were exposed to 98℃ water for 15 s which led to the third degree, 30% TBSA burns, while the rats in the sham group were exposed to 37℃ water for 15 s. Rats in burn group and AMSCs group were given 0.9% 10 mL (50 mL/kg) saline intraperitoneally immediately after burns. PBS or AMSCs were injected through caudal vein 3 hours later. 48 hours later, rats were sacrificed and the myocardium and blood from aorta abdominalis were collected. The levels of creatine kinase (CK), lactic dehydrogenase (LDH) were detected by ELISA. The mRNA levels of caspase-3, TNF-α, IL-1β and IL-10 were detected by RT-PCR. The data were analyzed through AVONA and LSD-t test by SPSS. Results The positive rates of CD29, CD44, CD90, CDl05 were very high in the third generation of AMSCs, however, the positive rate of CD34 was low. AMSCs underwent osteogenic and adipogenic differentiation after induction. The levels of CK and LDH in rats of the burn group were significantly increased compared with the sham group (P < 0.05). The levels of CK and LDH in AMSCs group were significantly lower than those in the burn group (P < 0.05). The mRNA levels of caspase-3, IL-1β and TNF-α in the myocardium of the burn group were significantly increased compared with the sham group (P < 0.05). The mRNA levels of caspase-3, IL-1β and TNF-α in myocardium of the AMSCs group were significantly lower than those in the burn group (P < 0.05). The mRNA level of IL-10 in the burn group was significantly lower than that in the sham group (P < 0.05). In the AMSCs group, the expression of IL-10 mRNA was significantly higher than that in the burn group (P < 0.05). Conclusion AMSCs can protect against myocardial injury induced by severe burns. The levels of CK and LDH decreased, indicating that the injury of myocardial decreased. During which, the inflammatory factors, IL-1β and TNF-α mRNA decreased and anti-inflammatory factor IL-10 mRNA increased.
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