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| Expression level and clinical significance of serum lncR-MEG3 in patients with acute pancreatitis |
| ZHANG Yun CHEN Wenxiao ZHANG Yan |
| Department of Gastroenterology, Taizhou First People′s Hospital, Zhejiang Province, Taizhou 318020, China |
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Abstract Objective To investigate the expression level and clinical significance of long non-coding RNA-MEG3 (lncR-MEG3) in serum of patients with acute pancreatitis. Methods Form March 2015 to January 2018, 97 cases of patients in Taizhou First People′s Hospital ("our hospital" for short) with acute pancreatitis were sollected as treated group, and it can be divided into patients with mild acute pancreatitis (MAP group, n = 62) and patients with severe acute pancreatitis (SAP group, n = 35), and from the same period were randomly selected 30 cases of healthy check-up for a control, using real-time fluorescent quantitative PCR method established groups of serum lncR-MEG3 expression level, and analyze its relationship with the clinical features and diagnostic value of illness severity of acute pancreatitis. Results The expression levels of lncR-MEG3 in serum of patients in MAP group and SAP group were lower than those in the control group, and those in SAP group were lower than those in MAP group (P ＜ 0.05). The improved CT severity index (MCTSI) score ≥4 points, bedside Acute pancreatitis severity bedside index (BISAP) score ≥3 points, Ranson score ≥3 points, and serum lncR-MEG3 expression levels in patients with acute pancreatitis were lower than MCTSI score＜ 4 points, BISAP score ＜ 3 points, and Ranson score＜ 3 points. The curve area (AUC) under ROC curve was used to evaluate the diagnostic value of serum lncR-MEG3 for the severity of acute pancreatitis. The results showed that the AUC was 0.805, 95%CI (0.720, 0.891), and the optimal cut-off value of lncR-MEG3 was 3.67, and the sensitivity and specificity were 0.79, 0.85, and accuracy was 0.82, respectively. Conclusion Serum lncR-MEG3 is involved in the pathogenesis of acute pancreatitis through down-regulation of its expression, which is closely related to the severity of acute pancreatitis. Early detection can be used as an important marker to evaluate the severity of acute pancreatitis.
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