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| Influence of Tiaowei Chengqi Decoction on the immunologic function of rats with gut-derived sepsis |
| ZHAO Fengli1 WANG Shuxin2 LUO Yuanyuan1 ZHENG Shuming1 CHEN Jing1 ZHAO Fu1 XIAN Shaoxiang3 |
1.Intensive Care Unit, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China;
2.Acupuncture Department, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China;
3.the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510405, China |
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Abstract Objective To observe the influence of Tiaowei Chengqi Decoction (TWCQ) on the immunologic function of rats with gut-derived sepsis. Methods Sixty SPF male SD rats were randomly divided into sham operation group, model group, Ulinastatin group (30 000 U/kg), TWCQ gavage group (9.45 g/kg), TWCQ enema group (9.45 g/kg) and TWCQ enema + Ulinastatine group according to their body weight (n = 8). After modeling for 2, 10, 24 h, Ulinastatine was administered by intraperitoneal injection, respectively. TWCQ was administered by gastric way and enema way respectively according to different groups, and the experiment was completed at 24 hours. The levels of serum interleukin 10 (IL-10) and tumor necrosis factor α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA), the levels of CD4+ and CD8+ T lymphocyte in mesentery of small intestine were detected by flow cytometry, and the results of HE staining in small intestine were observed under light microscope. Results Compared with sham operation group, the proportion of CD4+/CD8+ T lymphocyte in mesentery of small intestine in rats of model group was decreased significantly (P ＜ 0.05), the level of IL-10 was reduced and TNF-α was improved significantly (P ＜ 0.05), and the pathological damage of small intestine could be seen obviously. Compared with model group, the proportion of CD4+/CD8+ T lymphocyte in mesentery of small intestine in rats of TWCQ treatment group increased significantly (P ＜ 0.05), the level of IL-10 was improved and TNF-α was reduced significantly (P ＜ 0.05), and the damage degree of model small intestine was improved obviously. Compared with TWCQ gavage group, the therapeutic effect of TWCQ enema group was more obvious (P ＜ 0.05). Conclusion TWCQ has the function of improving the proportion of CD4+/CD8+ T lymphocyte and reducing immunologic function in model rats with gut-derived sepsis, and enema therapy is superior to gavage therapy.
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